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Portage Learning: Microbiology, Module 3 – MICROSCOPY |Questions with 100% Correct Answers | Updated & Verified | 2024
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micrometer - one millionth of a meter nanometer - one billionth of a meter
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Portage Learning: Microbiology, Module 3 – MICROSCOPY |Questions with 100% Correct Answers | Updated & Verified | 2024 micrometer - ✔✔one millionth of a meter nanometer - ✔✔one billionth of a meter resolution and contrast - ✔✔two critical factors that influence our ability to see an object resolution - ✔✔the distance between two objects at which the objects still can be seen as separate greater - ✔✔the closer two objects are to each other the __________________ the resolution requirement contrast - ✔✔the difference in light absorbance bet ween two areas (objects) lower - ✔✔the ________________ the contrast between an object and its background, the harder it will be to see the object bright field microscope - ✔✔simplest form of light, or optical, microscopy/light, most often emitted from a standard halogen bulb, enters the microscope from the base (bottom) and is reflected via mirrors towards the sample condenser - ✔✔before the light reaches the sample, it first passes through a ________________ converging the light beams into a focused ar ea on the sample iris diaphragm - ✔✔controls the amount of light that passes through the sample and into the objective lens objective lens - ✔✔the lens closest to the sample and yields the greatest amount of magnification directly proportional - ✔✔the d egree of magnification is ______________________ to the amount of light needed/to image samples clearly at higher magnifications, more light is required ocular lens - ✔✔eyepiece 10x - ✔✔most common power of ocular lens total magnification - ✔✔objective x ocular lens= staining - ✔✔required due to the limitation of resolution on unstained cells, (i.e.) often the flat and transparent regions of a cell may appear invisible under bright field conditions/with various dyes, th ese regions can become labeled and thus visualized. phase contrast microscope - ✔✔able to visualize certain structures that would otherwise be invisible/ can provide detailed images of live cells without staining./uses specialized condensers and objective s, it amplifies the slight differences between cells and the surrounding medium (background) to make the cells highly distinguishable/can be used to visualize cell movementsThe benefits of buying summaries with Stuvia:
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