Gene Expression—Transcription How Is MRNA Synthesi
Gene Expression—Transcription How is mRNA synthesi
Exam (elaborations)
Gene Expression—Transcription How is mRNA synthesized and what message does it carry? Why?
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Gene Expression—Transcription How is mRNA synthesi
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Gene Expression—Transcription How Is MRNA Synthesi
Gene Expression—Transcription
How is mRNA synthesized and what message does it carry?
Why?
DNA is often referred to as a genetic blueprint. In the same way that blueprints contain the instructions
for construction of a building, the DNA found inside the nuclei of cells contains the instructi...
Gene Expression—Transcription
How is mRNA synthesized and what message does it carry?
Why?
DNA is often referred to as a genetic blueprint. In the same way that blueprints contain the instructions
for construction of a building, the DNA found inside the nuclei of cells contains the instructions for
assembling a living organism. The DNA blueprint carries its instructions in the form of genes. In most
cases the genes direct the production of a polypeptide, from which other more complex proteins, such as
enzymes or hormones, may be constructed. These polypeptides and other molecules run the organism’s
metabolism and, in multicellular organisms, dictate what each cell’s job is. So, what is the language of
these instructions and how are they read and decoded by the cellular organelles? This activity will focus on
the decoding of genes in eukaryotes.
Model 1 – Transcription
5´ 3´
Chromosome from a
nondividing cell
Inside the Nucleus
Template (reading) strand
A
C
U
U
A
Outside the Nucleus
RNA polymerase and G
transcription factors
A
C
G
G
U
A
G
C
T
U
A
A
G
C
U
C
A
G
C
U
U
A
A
G
U
G
C
U
A
G
Direction Growing strand of pre-mRNA
of synthesis
G G U
C
C C
Free RNA Nuclear membrane
nucleotides
5´ 3´
Gene Expression—Transcription
This study source was downloaded by 100000865194348 from CourseHero.com on 04-28-2023 14:31:50 GMT -05:00
1
, 1. Consider the eukaryotic cell in Model 1.
a. Where in the cell is the DNA found?
b. Where in the cell does transcription take place?
2. Refer to Model 1.
a. What polymer is synthesized during transcription?
b. What monomers are used to construct this polymer and where are they found?
3. According to Model 1, what enzyme is required for transcription? (Hint: Think about how
enzymes are named. What ending is used for enzyme names?)
4. Refer to Model 1.
a. What is the base-pair rule for a DNA strand matching an RNA strand?
b. Compare this base-pair rule with that of two DNA strands.
5. Which strand of the DNA contains the “blueprint” for the pre-mRNA?
6. Consider Model 1.
a. In which direction is the DNA molecule read?
b. The DNA strand and pre-mRNA strand are anti-parallel. With this in mind label the 3ʹ and
5ʹ ends of the pre-mRNA strand in Model 1.
c. In which direction is the pre-mRNA molecule constructed?
7. Before printing presses were available, books had to be transcribed in order to share the informa-
tion in them. Consider the definition of transcription and explain why the process in Model 1 is
described using that word.
2 POGIL™ Activities for AP* Biology
This study source was downloaded by 100000865194348 from CourseHero.com on 04-28-2023 14:31:50 GMT -05:00
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