ASCP MLS EXAM 2023/2024 LATEST
UPDATE / ASCP MLS EXAM QUESTIONS
AND CORRECT
ANSWERS GUARANTEED A PASS
What feature is most often found with patients with a splenectomy on a Wright's stained
blood smear? - ANSWERS-Howell-Jolly bodies
Patients with antibody to the following antigen are immune to Hepatitis B: - ANSWERS-
Surface antigen (HbSAg)
Which two of the following tests are helpful for documenting previous Streptococcal
throat and skin infections: - ANSWERS-ASO titer and Anti-DNase B
In mass spectrometry, the "appearance potential" is defined as the: - ANSWERS-
Minimum energy required for the appearance of a particular fragment ion in mass
spectrum
What is generally accepted as the lower threshold value for semen pH from fertile
males? - ANSWERS-7.2
Coulometry: - ANSWERS-An automated method for measuring chloride which
generates silver ions in the reaction
Erythroleukemia: - ANSWERS-Also known as Acute Myelogenous Leukemia type M6.
This type is associated with either the presence of both erythroid and myeloid
precursors OR strictly erythroid precursors.
,Myelomonocytic leukemia - ANSWERS-Is associated with an increase in myeloid and
monocytic cell precursors, but not red blood cell precursors
Species that are Lysine (+), arginine (=) and ornithine (+): - ANSWERS-Enterobacter
aerogenes, Edwardsiella species and most Serratia species
Lysine reaction for all Citrobacter: - ANSWERS-Negative
Key identifying characteristic for Pantoa (Enterobacter) agglomerans: - ANSWERSnegative
reactions for the three decarboxylases commonly tested ( Lysine, arginine and ornithine)
Which organism is MOST likely responsible for septic reactions associated with Red Blood Cell
transfusions? - ANSWERS-Yersina entercolitica is most likely responsible for septic reactions in
transfusions of Red Blood Cells. This organism is usually acquired by ingestion of contaminated
food and causes mild symptoms of abdominal pain and diarrhea. Growth of Y. entercolitica is
enhanced in iron-rich environments such as red cell components.
Gaucher's disease - ANSWERS-The most common lysosomal storage disease caused
by insufficient activity of the lysosomal enzyme glucocerebrosidase that leads to the
deposition of glucocerebroside in cells of the macrophage-monocyte system.
Diseases associated with dysfunctions of polymorphonuclear neutrophils (PMNs) - ANSWERS-
Chediak-Higashi syndrome (CH), Chronic granulomatous disease (CGD), Myeloperoxidase
deficiency (MPO)
Nephrotic Syndrome: - ANSWERS-Caused by damage to the kidneys, especially the basement
membrane of the glomerulus; which causes abnormal excretion of protein and red blood cells in
the urine. Fats are also present in the urine in most cases. A foamy appearance of the urine is a
key characteristic of this condition.
,Clinical symptoms include:
Swelling
Weight gain from fluid retention
Poor appetite
High blood pressure
Key Biochemical reactions for Enterobacteriaceae: - ANSWERS-The key biochemical reactions
by which the family Enterobacteriaceae can be identified include fermentation of carbohydrates,
reduction of nitrates to nitrites and the absence of cytochrome oxidase activity.
ANA - ANSWERS-See 2 quiz #27
Prozone effect: - ANSWERS-Results in a false positive reaction; is the result of antibody
excess and dilution of antibody can help prevent its occurrence
Purpose of a bi-chromatic analyzer with dual wavelengths is: - ANSWERS-Minimize the
effect of interference
Key characteristics of Micrococcus: - ANSWERS-Susceptible to bacitracin ("A") and
resistant to furazolidone; gram positive cocci in tetras, bacteria cells of micrococcus
luteus are larger than those of the staphylococci and can arrange in tetras
What is the glomerular filtration rate for a patient with a serum creatinine of 2 mg/dL, if
the urine creatinine was 124 mg/dL and the urine volume was 2.2 L/24 hrs? -
ANSWERS-95 mL/min
One method of calculating a glomerular filtration rate is using creatinine and urine
volume to determine creatinine clearance. The equation is as follows:
Creatinine Clearance = (urine creatinine X urine flow rate) / plasma creatinine; where
urine flow rate = volume in mL /24 hours x h/60 min)
, In this case = creatinine clearance = 124 X (2200/24 x hour/60) / 2 = 94.7 or 95 ml/min
Estrogen and progesterone markers are most commonly used to provide prognostic
information about: - ANSWERS-Breast cancer
The prozone effect ( when performing a screening titer) is most likely to result in: - ANSWERS-
Prozone effect (due to antibody excess) will result in an initial false negative in spite of the large
amount of antibody in the serum, followed by a positive result as the specimen is diluted.
Which of the following Rh antigens is found the highest frequency in the Caucasian
population:
A. C
B. E
C. c
D. e - ANSWERS-D. e
The accuracy of an immunoassay is its ability to discriminate between results that are true
positives and results that are true negatives. Two parameters of test accuracy are specificity and
sensitivity. Which of these statements apply to an immunoassay with high specificity, but low
sensitivity? - ANSWERS-Accurately identifies the absence of disease and has few false-positives
A test with high specificity accurately detects the absence of disease. The more specific a test is,
the fewer false-positive results will occur. A test with high sensitivity accurately identifies the
presence of disease. The more sensitive a test, the fewer false-negative results it produces. In the
case stated in this question, the immunoassay has high specificity, so it has few false-positives
and will accurately detect those individuals who do not have the disease or condition that is being
tested for. However, the test has low sensitivity, so it may not identify all individuals who
actually have the disease; it may produce many false-negative results.
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