ASCP MB EXAM 2023/2024 LATEST WITH
QUESTIONS AND ANSWERS 100% CORRECT
NEWEST UPDATE (RATED A+)
Consider the table below where a child and a possible father (PF) share the listed
paternity indices for each locus listed (LOC-A1, LOC-B2, LOC-C3, LOC-D4).
Based on the data presented in the table, what is the combined paternity index, CPI,
from the loci tested: LOC-A1, LOC-B2, LOC-C3 and LOC-D4?
12.42
9.558
15.56
2.38 - ANSWERS-12.42 ( CPI is calculated by multiplying all Paternity index tgt)
Best way to avoid PCR contamination - ANSWERS-Separate Pre-PCR (clean area) and
post PCR (dirty area)
A260=1.4, A280=1.1 . protein contamination or Rna contam or useable RNA -
ANSWERS-Protein contamination . A260/280 = 1.3
<1.6 = protein contamination, 1.7-2.0 = good DNA, 1.9-2.0 = good RNA
,Why is Fluorometry a better technique that Spectroscopy - ANSWERS-Increased sensitivity and
more importantly can accurately measure DNA concentration of a structurally intact double
stranded DNA molecule
Linkage Disequilibrium - ANSWERS-LD is the non random association of alleles at 2 or
more loci in a general population. When alleles are in LD, haplotypes do not occur at
the expected frequencies.
Limit of Detection (LOD) - ANSWERS-lowest concentration of analyte that can be
detected
mRNA Sequence provided - Convert it into DNA - ANSWERS-(Template/Anti-sense
Strand) [Replace U with T]
Correct order of HIV Sample Preparation Workflow - ANSWERS-Plasma Preparation
RNA Extraction
RNA Amplification/Modification Procedure
If a flask of cells is provided for the intended purpose of DNA isolation, how would you
proceed further - ANSWERS-Trypsinize and/or scrape the cells inside the provided
flask, collect the cells suspended in the media, spin, and then proceed further
Which end of the primer is critical for the increased specification of PCR Amplification
process - ANSWERS-3'End of the Primer
Primer Dimer Complementarity - ANSWERS-5' End of the Primers (3-3' Was not an
option)
Mechanism of Action of MecA Gene in MRSA - ANSWERS-Altered Drug Target
,(PBP2A)
Technique to be used for FFPE Cervical Tissue Provided - ANSWERS-Invader Assay
(Non-Amplification Method)
Liquid Based-Cytology Useful for what kind of virus - ANSWERS-HPV is the correct
answer (Options Provided for: HIV, HBV, HCV, HPV)
Isothermal Method that utilizes Primes and Polymerase to Create Synthetic dsDNA -
ANSWERS-SDA (Strand Displacement Amplification)
Polymerase used in Isothermal LAMP Procedure - ANSWERS-BST (Bacillus
Stearothermophilus)
BRAF V600E Mutation is Associated with - ANSWERS-Lynch Syndrome (Also Known
as HNPCC)
CAP Requires Discontinued SOP/Procedures to be stored for how long - ANSWERS-At
least 2 years from the date of discontinuation
CAP Requires Laboratory Personnel (Technologist) Proficiency Testing - ANSWERS-
Once a Year
Most Important Component of Clinical Laboratory Completed Validation Studies -
ANSWERS-Final Test Validation Summary Signed off by Laboratory Director
STR Electropherogram provided and select the most informative loci - ANSWERS-Two
different alleles each was both recipient vs donor the most informative one
STR makes use of which of the following - ANSWERS-Variable Nucleotide
, Experiment Performed (RFLP + PCR + Gel Electrophoresis): three bands observed in the mutant
sequence as compared to the normal sequence - ANSWERS-Insertion of New Restriction Site
Mutation in what gene decreases metabolism of Irinotecan Drug - ANSWERS-UGT1A1
CYP2D6 Enzyme Metabolizes what drug - ANSWERS-Codeine
Determine Melting Temperature of a given DNA Sequence - ANSWERS-2(A+T) +
4(G+C)
Specimen Collection for Testing of Cystic Fibrosis - ANSWERS-Either 1 ml EDTA Lavender
Tube/5 ml ACD Tube (Not Sure: I think I answered 1 ml EDTA, however the volume seems to be
too low)
Fish is used in - ANSWERS-Prader Willi Syndrome
Defects associated with Prader Willi Syndrome - ANSWERS-Uniparental Disomy and
Genomic Imprinting - Paternal Chromsome Deletion 15
Which of the Following is Trinucleotide Repeat Expansion Disorder - ANSWERS-
Huntington's Disease, Fragile X
HER2/EGFR2 Overexpression - ANSWERS-Trastuzumab Therapy
Overexpression of estrogen, so treat with Herceptin/Tamoxifin Trastuzumab therapy
T-Cell Receptor Gene Rearrangement - ANSWERS-Hodgkin Lymphoma (PS: Don't get
confused with T-Cell Lymphoma that corresponds to Sezary Syndrome, both options
will be present for confusion)
Genes Involved in the Cell Cycle Process are termed as - ANSWERS-Cycle-Dependent
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