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ASCP MB EXAM 2023 NEW UPDATE WITH SOLUTIONS 100% CORRECT/ASCP REAL EXAM (RATED A+)

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RNA Polymerase I - ANSWERS-DNA template Location: Nucleous Products: 18s, 5.8s, 28s rRNA RNA Polymerase II - ANSWERS-DNA template Location: Nucleus Products: mRNA, snRNA RNA Polymerase III - ANSWERS-DNA template Location: Nucleus Product: tRNA, 5s rRNA, snRNA ...

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  • January 8, 2024
  • 36
  • 2023/2024
  • Exam (elaborations)
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ASCP MB EXAM 2023 NEW UPDATE WITH
SOLUTIONS 100% CORRECT/ASCP REAL EXAM
(RATED A+)
.


E. coli RNA Polymerase II - ANSWERS-DNA template, product: mRNA


RNA Polymerase I - ANSWERS-DNA template
Location: Nucleous
Products: 18s, 5.8s, 28s rRNA


RNA Polymerase II - ANSWERS-DNA template
Location: Nucleus
Products: mRNA, snRNA


RNA Polymerase III - ANSWERS-DNA template
Location: Nucleus
Product: tRNA, 5s rRNA, snRNA


Mitochondrial RNA Polymerase - ANSWERS-DNA template, product: mRNA


Mammalian DNA polymerase α - ANSWERS-DNA template, product: primers


HCV RNA Polymerase - ANSWERS-RNA template, product: viral genome


Dengue virus RNA Polymerase - ANSWERS-RNA template, product: viral genome


Poly A Polymerase - ANSWERS-No template, product: Poly A tail

,230 wavelength - ANSWERS-Contamination of organic compounds


270 wavelength - ANSWERS-Phenol contamination


280 wavelength - ANSWERS-Protein contamination


>330 wavelength - ANSWERS-Particulate matter contamination


Southern Blot - ANSWERS-DNA Target
Nucleic acid probe
Purpose: Paternity testing, gene discovery and mapping, mutation analysis and ID, gene structure


Northern Blot - ANSWERS-RNA Target
Nucleic acid probe
Purpose: Determine transcript structure, processing, and gene expression


Western Blot - ANSWERS-Protein Target
Protein probe
Purpose: Protein processing, gene expression


Southwestern Blot - ANSWERS-Protein target
DNA probe
Purpose: DNA binding proteins, gene regulation


Eastern blot - ANSWERS-Protein target
Protein probe
Purpose: Modification of Western blot using enzymatic detection (protein processing, gene
expression), detection of specific agriculturally important proteins


Far-eastern blot - ANSWERS-Lipid target
No probe

,Purpose: Transfer separated lipids to membranes for analysis by mass spectometry


PCR Primers - ANSWERS-Direct DNA synthesis to desired region


PCR: dATP, dCTP, dGTP, dTTP - ANSWERS-Building blocks that extend primers


PCR KCl - ANSWERS-Salt for optimal hybridization of primers to template


PCR: Tris buffer - ANSWERS-Buffer to maintain optimal pH for enzyme reaction


PCR: MgCl2 - ANSWERS-Divalent cation, required by enzyme


PCR Polymerase - ANSWERS-Polymerase enzyme that extends the primers, adds the
dNTPs


PCR: copies of template - ANSWERS-Sample DNA that is being tested


Denaturation temps/time - ANSWERS-90-96, 20-60 sec


Annealing temps/time - ANSWERS-50-70, 20-90 sec


Extension temps/time - ANSWERS-68-75/10-60 sec


+ - ANSWERS-gain


- - ANSWERS-loss


del - ANSWERS-deletion


der - ANSWERS-derivative chromasome

, dup - ANSWERS-duplication


ins - ANSWERS-insertion


inv - ANSWERS-inversion


i, iso - ANSWERS-isochromosome


mat - ANSWERS-maternal origin


pat - ANSWERS-paternal origin


r - ANSWERS-ring chromosome


t - ANSWERS-translocation


tel - ANSWERS-telomere (end of chromosome arm)


Bioinfo: Identity - ANSWERS-Extent to which 2 sequences are the same


Bioinfo: Alignment - ANSWERS-Lining up 2+ sequences to search for maximal regions
of identity in order to assess extent of relatedness/homology


Bioinfo: Local alignment - ANSWERS-Alignment of some portion of 2 sequences


Bioinfo: Multiple sequence alignment - ANSWERS-Alignment of 3+ sequences arranged
with gaps so that common residues are aligned together


Bioinfo: Optimal alignment - ANSWERS-Alignment of 2 sequences with best degree of
identity

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