Thin Layer Chromatography –Questions/Answers
____ a method of separating mixtures into their pure components ✔️Ans -
Chromatography
chromatography: a method of _____ ✔️Ans - separating mixtures into their
pure component
Chromatography separates mixtures because the ____ between the various
components of the mixture and the stationary phase are different ✔️Ans -
intermolecular attractions
Chromatography separates mixtures because the intermolecular interactions
between the ____ ✔️Ans - various components of the mixture and the
stationary phase are different
Chromatography is a method of separation using a ___ and a ____ ✔️Ans -
stationary phase; mobile phase
separation occurs as the mobile phase ____ the stationary phase ✔️Ans -
passes over
the mobile phase can be ___ or ___ ✔️Ans - liquid; gas
the stationary phase can be ___ or ___ ✔️Ans - solid; liquid
Chromatography-Basic Principle: same basic principle as TLC but now ____ of
pressure (_____) to ___ compounds ✔️Ans - use gravity; medium to high;
elute
Chromatography is both a ____ and ____ method ✔️Ans - qualitative;
quantitative
Chromatography is used for _____ the course of a reaction ✔️Ans -
monitoring
Chromatography is used for monitoring ____ ✔️Ans - the course of a
reaction
, 3 types of chromatography used for monitoring the course of a reaction
✔️Ans - thin layer chromatography; gas chromatography; high performance
liquid chromatography
what does TLC stand for ✔️Ans - thin layer chromatography
what does GC stand for ✔️Ans - gas chromatography
what does HPLC stand for ✔️Ans - high performance liquid
chromatography
chromatography is used for _____ the components of a reaction ✔️Ans -
isolating
what methods of chromatography are used for isolating the components of a
reaction ✔️Ans - column chromatography; prep HPLC; Prep TLC
Chromatography is both a qualitative and quantitative method used for ___, ___
and ___ ✔️Ans - 1) monitoring the course of a reaction
2) isolating the components of a reaction
3) assisting in the identification of a compound
In liquid chromotagraphy ____ is established ✔️Ans - equilibrium
equation for chemical equilibrium is established and can be represented by
the following equation ✔️Ans -
in the equation for chemical equilibrium what does Xm and Xs stand for
✔️Ans - the concentrations of solute molecules in the mobile phase and the
stationary phase
in the equation for chemical equilibrium what does Sm and Ss stand for
✔️Ans - Sm and Ss are for the solvent molecule concentrations
in the equation for chemical equilibrium what does the value K represent
✔️Ans - the distribution coefficient or equilibrium between the solute and
solvent molecules and between the mobile and stationary phases
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