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PAPER CHROMATOGRAPHY LAB REVIEW – Q&A
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PAPER CHROMATOGRAPHY LAB REVIEW – Q&A
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PAPER CHROMATOGRAPHY LAB REVIEW – Q&AAll 7 dyes have ✔️Ans -Conjugated systems
Conjugated systems ✔️Ans -Long carbon structures with alternating
carbon-carbon single and double bonds
Lots of conjugated bonds means ✔️Ans -Color
Chromatography ✔️Ans -Group of techniques used to separate colored
mixtures into their component parts (often used for blood or drinking water
analyses)
Chromatography involves ✔️Ans -A stationary phase and a mobile phase
Stationary phase can be ✔️Ans -Solid or liquid
The mixture to be separated is placed ✔️Ans -On the stationary phase
Mobile phase can be ✔️Ans -Liquid or gas
Liquid chromatography ✔️Ans -The separation is based on preferential
attraction of each component of the mixture to either stationary or mobile
phase
The mobile phase moves along the stationary phase, ✔️Ans -Carrying some
or all of the mixture with it, resulting in the separation of the mixture
components
Attractions of components to mobile or stationary phase is due to ✔️Ans -
Intermolecular interactions
Paper chromatography ✔️Ans -Sample of mixture is put on
chromatography paper, which acts as stationary phase; edge of paper placed
in solvent, which acts as mobile phase
Wicking occurs because ✔️Ans -Solvent is attracted to water molecules
that are bound to cellulose fibers of paper
, In paper chromatography, the sample is ✔️Ans -Spotted on the origin line
Once sample has been spotted, ✔️Ans -Bottom edge of paper is put in
solvent and solvent moves up paper; when solvent front reaches sample, the
sample components are attracted to either stationary or mobile phase
Solvent front ✔️Ans -Leading edge of mobile phase
Attraction depends on what property? ✔️Ans -Relative polarities of sample
components and two phases
The attraction is never ✔️Ans -All or nothing
Equilibrium equation ✔️Ans -Component-mobile phase< --> component-
stationary phase
As fresh solvent continually passes the sample, ✔️Ans -New equilibria are
established
Even as new equilibria are established, ✔️Ans -Any components that have
dissolved in the mobile phase encounter fresh stationary phase and new
equilibria are established
Components of mixture move ✔️Ans -At different rates and separate,
creating chromatogram
Overall effect of these equilibria ✔️Ans -The movement of the components
depends directly on their relative attractions for mobile and stationary phases
Characterize movement of components in terms of a ✔️Ans -Retention
factor (Rf)
Retention factor equation ✔️Ans -Distance traveled by
component/distance traveled by solvent front (cm)
Highest possible Rf? Lowest? ✔️Ans -1.0, 0.0
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