Summary
Samenvatting H9: Somitogenesis and Limb development - Prezmko - Developmental Biology
- Course
- Institution
Volledige samenvatting H9: Somitogenesis and Limb development - Developmental Biology
[Show more]
1 review
By: estherviaene • 1 month ago
Seller
Follow
lukanys11
Reviews received
Content preview
Somitogenesis and limb developmentSomitogenesis
Somites form from unsegmented mesoderm.
Here we look at chicken embryo in different
stages from the dorsal view.
A. Earlier stage, not much to be seen
besides the node and some primitive
streak.
B. Here you can start to see something,
beginning of the somites
C. Here you start seeing the somites.
Look at different dissections:
- Primitive streak
- Epiblast → ectoderm
- Endoderm
- Migrating mesodermal cells → EMT
- Paraxial mesoderm
- We are above the node → see full picture of embryo above
- Somites
- Somatic mesoderm
This is what it looks like in a schematic way. Look at primitive streak and
direction of elongation.
1
,Here we look at the development of the mesoderm:
We talk about the paraxial mesoderm and
specifically the somites today.
Who becomes who?
This part of somites gives rise to the muscles in the limbs.
2
, Traditional view is how it was viewed back then, but now we know more!
Somitogenesis → formation of the somites.
BrdU-staining = cell proliferation (methods!!)
We do not know how left and right side somites know they have to form together as they are
physically separated.
Fibronectin → red staining of membrane.
How come the somites form → molecularly.
Looking at different expressions:
- Mesp is a target of Notch (see signalling pathway).
In situ hybridization
What different information do we get from exonic and intronic in situ hybridization?
- where mRNA is present → where the translation and protein are highly likely to also be.
- where transcription takes place
3
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller lukanys11. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $7.47. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
73314 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now