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MIBO 3500 EXAM 1 WITH 100% CORRECT ANSWERS
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MIBO 3500 EXAM 1 WITH 100% CORRECT ANSWERS
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MIBO 3500 EXAM 1 WITH 100%CORRECT ANSWERS
microbe - answer living single-celled organism that requires a microscope and has the capacity
to reproduce
*some microbes can be seen without a microscope*
naming system used:________;
1st word: _________________; always ____________;
2nd word: _______________;
full name is always: ____________ - answer binomial;
genus --> capitalized;
specific epithet;
italicized or underlined
Who built the compound microscope and coined the term "cell"? - answer Robert Hooke
Who first observed single-celled microbes? - answer Antonie van Leeuwenhoek
spontaneous generation - answer living creatures could arise without parents
*rejected by many scientists
,Who demonstrated that microbes cause fermentation and food spoilage and is known as the
father of MIBO? - answer Louis Pasteur
importance of swan-neck flasks - answer determined that diseases have identifiable causes
Who configured the 3 domains? (eukarya, archaea, and bacteria) - answer Carl Woese
Magnification of light microscope - answer 1000x
what happens in a light microscope? (bright-field microscope) - answer light passes thru
specimen --> then thru a series of magnifying lenses
magnification of electron microscope - answer 100,000x
magnification of scanning probe microscope - answer view at atomic level
wavelength of visible light - answer 400 - 750 nm
absorption - answer photon's energy is acquired by the absorbing object
reflection - answer a wavefront bounces off the surface of an object
refraction - answer bending of light as it enters a substance that slows its speed
scattering - answer a wavefront interacts with objects smaller than the wavelength of light
the 3 elements of light microscopy - answer magnification, resolution, contrast
, magnification - answer the ratio of the size of an object to the size of its image --> image is seen
as larger than object
-refracts light to spread its waves (bends the light precisely)
components of a compound microscope - answer 2 magnifying lenses:
ocular lens (10x) and objective lens (greater than 100x)
total magnification is product of ocular and objective lenses
resolution - answer ability to resolve two objects very close together;
quantified as the minimum distance between two objects where they still appear separate
what does resolution depend on? - answer quality of lens and wavelength of light
empty magnification - answer magnification without increasing detail --> results in highly
pixellated image
what is the detail of an image limited by? - answer the wave nature of light --> light rays
undergo interference
higher quality lens = better resolution = ____________ - answer higher Numerical Aperture
name a method that increases resolution - answer immersion oil method - decreases bending of
light since n(oil) is approximately equal to n(glass)
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