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JMU Bio 140 Lab Checkpoint Quiz 5-12 Questions and Answers (2023/2024) (Verified Answers)
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JMU Bio 140 Lab Checkpoint Quiz 5-12 Questions and Answers (2023/2024) (Verified Answers)
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JMU Bio 140 Lab Checkpoint Quiz 5-121.Why is it important to post-rinse when pipetting volumes less than
20 microliters?
A. Small volumes may not get expelled completely due to surface tension.
B. You can't accurately pipette less than 20 microliters.
C. You don't need to post rinse small volumes.
D. Small volumes will evaporate prior to transfer.: A.
2.Pipettors are most accurate in the middle to top end of their volume
range. True
False: True
3.How can contamination be prevented in the laboratory?
A. Keeping test tubes and pipette tip boxes closed as much as possible.
B. Changing pipette tips between solutions, or after tips have touched DNA.
C. Using barrier pipette tips.
D. Any of these methods will help prevent contamination in the laboratory.:
D.
4.When filling a pipette you should begin by depressing the plunger to the
in .: first stop, the air
,5.Next you should insert the tip into the liquid and let the plunger
out to prevent air bubbles and splash back.:
slowly
6.Once you remove the tip from the liquid, you should expel it by
depressing the plunger to the stop.:
quick- ly, second
7.L¼stands for: microliter
8.how many liters is a microliter?: 10^-6
9.45.3 µL (microliters) = mL (milliliters): .0453 mL
10.Test tubes should always be placed into a centrifuge with the
hinges facing inward.
True
False: False
11.The detergent and salt in DNA extraction buffer dissolve membranes
and dissociate proteins from the DNA.
True
False:
True
, 12.For the greatest accuracy in pipetting, one person should hold the
pipet- tor while a second person holds the test tube the person is pipetting
into or out of.
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