ASCP Recalls
Subject: Microbiology
1. Identify the organism: Bile esculin: POSITIVE; 6.5% NaCl: POSITIVE; PYR: NEGATIVE; LAP: NEGATIVE
Ans: Leuconostoc
2. A gram stain from a blood agar shows gram positive cocci in chains, non-hemolytic, The bacteria
grow on 6.5% NaCl and is Bile esculin posit...
ASCP Recalls Subject: Microbiology 1. Identify the organism: Bile esculin: POSITIVE; 6.5 % NaCl: POSITIVE; PYR: NEGATIVE; LAP: NEGATIVE Ans: Leuconostoc 2. A gram stain from a blood agar shows gram positive cocci in chains, non -hemolytic, The bacteria grow on 6.5% NaCl and is Bile esculin positive. What’s the most likely identification? Ans: Enterococcus ( only Enterococcus can grow both 6.5% NaCl and Bile Esculin ) 3. latex agglutination for Staphylococcus aureus: Ans: Protein A and clump ing factor 4. Pink mucoid colonies on MACTSI=A/A Spot indole test: POSITIVE Ans: Klebsiella oxytoca 5. A patient is diagnosed wit streptococcal pharyngitis 6 months ago. Now he is showing signs of glumerulonephritis. What will be seen in the renal b iopsy? Ans: S. pyogenes ( S. pyogenes is the most common causative agent for pharyngitis) 6. Biochemical reaction: Bile esculin: positive; 6.5% NaCl: negative Ans: Streptococcus bovis ( 6.5% NaCl test along with Bile -Esculin test, is used in many laboratories to distinguish Enterococcus species from the group D streptococci, Streptococcus bovis and Streptococcus lactis ) 7. Which of the following tests cab differentiate Pseudomonas aeruginosa from Pseudomonas putida? Ans: Growth at 42 degrees Celsius ( P. aeruginosa had the widest range of growth temperature 25 -42°C ) 8. Favours growth of anaerobic gram negative bacilli: Ans: Vitamin K and hemin 9. which of the following tests should be done first in order to differentiates Aerom onas spp from the Enterobacteriaceae? Ans: Oxidase ( Aeromonas: TSI reaction: A/A, and is Oxidase Positive. Enterobacteriaceae is oxidase Negative ) 10. A patient with walking pneumonia is being treated with penicillin. State one possible condition why the patient is not recovering: Ans: The organism has no cell wall 11. Causative agent of “Cat scratch disease”? Ans: Bartonella henselae ( cat -scratch disease (bartonellosis) which, as the name suggests, occurs after a cat bite or scratch. The dise ase is characterized by lymphadenopathy) 12. Hair perforation test/hair baiting test is for identification of which two fungi? Ans: Trichophyton mentagrophyte and T. rubrum ( Trichophyton mentagrophyte is positive and T. rubrum is negative ) 13. Gentamycin resistant organism : Ans: Enterococci 14. Lysostaphin resistant organism: Ans: Micrococcus 15. Cat bite Fever: Ans: Pasturela multocida 16. What is the infective stag e of Le ishmania spp. To humans?? Ans: Promastigote 17. The third Taenia spp ?: Ans: T . asiatica 18. Which o f the fo llowing media identifies species of Aspergillus? Ans: Czapek ’s aga r 19. This pres umptive tes t for Candi da uses serum. Ans: Germ tube test
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