Summary All exercises/self study assigments and solutions from Genome Technology and Applications (19/20)
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Course
Genome technology and applications (2051FBDBMW)
Institution
Universiteit Antwerpen (UA)
All exercises/self study assigments and solutions from Genome Technology and Applications (19/20): This document includes all questions AND FULL answers to the exercises of the exercise session of Genome Technology and Applications. It has the answers given by professor Guy van Camp and is thus ver...
self study assignment More recent and
convenient cloning systems Genome
Technology and Applications
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Self study assignment: More recent and convenient cloning systems
Prof. Van Camp
Fundamentals of gene and genome technology
Gene and genome technology
Learning aids:
Several video’s and texts on Gateway cloning and Gibson assembly are available on Blackboard
Aims:
1) Gateway cloning
The student can describe the CcdA/CcdB selection system in cloning with technical details
The student can describe the Gateway cloning system with technical details and required
components
The student can describe the advantages and disadvantages of the Gateway cloning system
The student can list and explain the differences between Gateway cloning and traditional
cloning using restriction enzymes and ligase
The student can design a cloning experiment using the Gateway system (the student knows
which att sites to use in which stage of the cloning experiment)
2) Gibson assembly
The student can describe the Gibson assembly with technical details and required compenents
The student can describe the advantages and disadvantage of Gibson assembly
The student can incorporate Gibson assembly in the theoretical design of cloning
experiments to replace restriction enzyme/ligation steps
Gedownload door: InnePatteet | inne.patteet@hotmail.be
Dit document is auteursrechtelijk beschermd, het verspreiden van dit document is strafbaar.
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