Deze samenvatting van het vak medische immunologie bevat een overzicht van hoofdstuk 4 over de laboratoriumtechnieken in een immunologisch labo en een samenvatting met eigen figuren van H7 over de ontwikkeling en de activatie van lymfocyten.
H4: Immunologische
laboratoriumtechnieken
1. Agglutinatietechnieken
a. Hemagglutinatie
b. Latexagglutinatie
c. Bacteriële agglutinatie
2. Precipitatietechnieken
a. Vloeibare fase systemen
i. Turbidimetrie
ii. Nefelometrie
b. Vaste fase systemen
i. Radiale immunodiffusie
ii. Immuno-elektroforese
3. Biologische activiteit
a. Complement fixatie test
b. Virus neutralisatie test
c. Hemagglutinatie inhibitie test
4. Gemerkte antilichamen (vloeibaar)
a. Enzyme
i. Capture ELISA
ii. Competitieve ELISA
b. Radioactief isotoop
i. Competitieve RIA (RIST, PRIST, RAST)
ii. IRMA: immunoradiometrische analyse (=captrure ELISA)
c. Fluorescente groep
i. FIA (competitief en capture)
Bv. ISAC: Immuno Solid-phase Allergen Chip
d. Chemiluminescente groep
i. Chemiluminescentie
5. Gemerkte antilichamen (cellen)
a. Cellulaire assay (ELISA / FIA)
b. Flowcytometer
, 6. Gemerkte antilichamen (weefsel)
a. Directe methode
b. Tweestaps indirecte methode
c. Driestaps indirecte methode
d. Oplosbaar enzyme-immunocomplex (PAP – APPAAP)
e. (strept)avidine-biotine methodes
f. UltraAmp technologie
g. Dextraan polymeerketens
h. Biotynil-tyramide based detection systems
Thymus – thymocyten/T-celprecursor
1) Subcapsulaire schorszone
- Dubbel negatieve thymocyten CD4- en CD8-
- Geen TCR/CD3 complex: CD3-
STAP 1: vorming T-cel receptor (TCR)
- Onbeperkt aantal Ag
- Ag specifiek ≠ in Vα en Vβ domein
- TCR = eiwit gecodeerd door gen
! Genetische herschikking !
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller robbecnockaert. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $3.24. You're not tied to anything after your purchase.