HTL ASCP Exam- Nerve questions with
correct answers
Cresyl Echt Violet (Luna) method - Answer Nissl substances (basophilic) dyed, alcohol used to differentiate.
Cresyl Echt Violet (Vacca) method - Answer Nissl substances (basophilic) dyed, acetic acid used to differentiate.
Bodain Method for Nerve Fibers - Answer Protargol (silver solution) with added COPPER destains connective tissue. Hydroquinone reduces, gold chloride, sodium
thiosulfate follows.
Holmes Method for Nerve Fibers - Answer Buffered silver solution + pyridine (alkali), reduced by hydroquinone, gold chloride, sodium thiosulfate.
Bielschowsky-PAS for Nerve Fibers - Answer Ammoniacal silver solution impregnation, formaldehyde reductions, gold chloride tones, sodium thiosulfate removes unreacted silver. PAS stains amyloid plaques and lipofucsin.
Sevier-Munger Modification - Answer Bielschowsky method modified by not using gold chloride to tone.
Mallory PTAH method for Glial fibers - Answer Phosphotungstic acid stains neurons while hematein stains glial fibers and nuclei.
Holzer method for Glial fibers - Answer Crystal violet, resist decolorization with the alkaline aniline- chloroform mixture.
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