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MB (ASCP) EXAM A+ GRADED
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MB (ASCP) EXAM 2023-2024 A+ GRADED______________ are identified by PCR product size - ✔STR alleles
2 of the holoenzymes are what? - ✔catalytic DNA polymerizing enzymes, one for
synthesis of the leading strand and one for the synthesis of the lagging strand
A gene contains what type of sequences? - ✔Structural sequences that code for an
amino acid sequence and regulatory sequences taht are important for the regulated
expresion of the gene.
A gene has what two types of sequences: - ✔regulatory and structural
A Robertsonian translocation may be associated with which genetic disorder? - ✔Down
Syndrome
A single protein can have separate _______ with _________ ___________ - ✔
domains, different properties
Acrocentric - ✔
Acute Lymphoblastic Leukemia - ✔t 17;19
Acute Myelogenous Leukemia - ✔t 1;12
Alpha Phosphate - ✔During RNA synthesis, the first ribonucleoside triphosphate retains
all of its phosphate groups as the RNA is polymerized in the 5' to 3' direciton.
Subsequent ribonucleoside triphosphates retain only the alpha phosphate, the one
closest to the ribose sugar.
Alternative splicing - ✔modifies products of genes by alternate insertion of different
exons
Amino acid that all proteins start with: - ✔Methionine
AML (Acute Myeloid Leukemia) - ✔t 12;15
An artifact observed in PCR which are double the size of primers. - ✔Primer dimer
Antibiotic resistance mechanism in MRSA? - ✔mecA > PBP2a resistance to methicillin
Associated with increased risk of SJS and toxic epidermal necrolysis in response to
carbamazepine treatment - ✔HLA-B*15:02
At one end of the peptide will be ________ and at the opposite terminus of the peptide
will be a _____. - ✔amino-terminal and carboxyl-terminal
,MB (ASCP) EXAM 2023-2024 A+ GRADED
ATG CAG GTG (M Q V)
ATG CAG GTT (M Q V) - ✔Silent
ATG CAG GTG (M Q V)
ATG CCG GTG (M P V) - ✔Missense
ATG CAG GTG (M Q V)
ATG CCG Stop (M Q) - ✔Nonsense
Best condition for long term storage of RNA? (6 months) - ✔-70C ethanol
Best method listed to collect bone marrow specimens: - ✔EDTA or ACD tubes
Blood should be stored and transported in: - ✔EDTA (purple/lavender top tubes) or
ACD (yellow top tubes)
Burkitt Lymphoma - ✔t 8;14
CGH arrays are performed on: - ✔DNA
Chaperones are proteins that - ✔help other proteins fold properly... begins to fold into
its mature conformation
Chromosome affected in Cri du chat: - ✔Deletion 5p
Chromosome affected in Digeorges Syndrome: - ✔Deletion 22q
Chromosome affected in Down Syndrome: - ✔Trisomy 21
Chromosome affected in Edward Syndrome: - ✔Trisomy 18
Chromosome affected in Gaucher Disease: - ✔1
Chromosome affected in Klinefelter Syndrome: - ✔Extra X
Chromosome affected in Patau Syndrome: - ✔Trisomy 13
Chromosome affected in Super Y: - ✔Extra Y
Chromosome affected in Tay Sachs Disease - ✔15
Chromosome affected in Turner Syndrome: - ✔Monosomy X
, MB (ASCP) EXAM 2023-2024 A+ GRADED
Clinical Sensitivity (SNIP) - ✔[TP/(TP+FN)] x 100
Clinical Specificity (SPIN) - ✔[TN/(TN+FP)] x 100
CML (Chronic Myelogenous Leukemia) - ✔t 9;22
codon - ✔three-nucleotide sequence on messenger RNA that codes for a single amino
acid
Concentration Formula - ✔Concentration = DNA/RNA constant x OD260 value x
Dilution factor
Conjugated proteins - ✔have components other than amino acids
conjugated proteins - ✔have components other than amino acids. THe nonprotein
cojmponent of a conjugated protein is the prosthetic group. Ex: lipoproteins, sugars,
mucin insaliva, and metalloproteins...HEMOGLOBIN
Constitutive Transcription - ✔Some messages are transcribed constantly and are
relatively abundant in the cell
Cut and reclose the DNA sugar-phosphate backbone without tangling - ✔Helicase
CYP2C9 - ✔Metabolizes warfarin
Detect changes in the RNA levels of specific genes - ✔Expression arrays
Detect deletions and amplifications of chromosomal regions - ✔CGH arrays
Detection Method/s for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG): -
✔Hybrid Capture, TMA, Hybridization protection assay, PCR, SDA
Detection Method/s for cytomegolovirus (CMV): - ✔NASBA, hybrid capture
Detection Method/s for HCV (qualitative): - ✔bDNA
Detection Method/s for HCV (quantitative): - ✔TMA, PCR
Detection Method/s for HIV (quantitative): - ✔bDNA, NASBA, RT PCR
Detection Method/s for HIV resistance testing: - ✔Sequencing
Detection Method/s for HPV: - ✔Hybrid capture
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