MB (ASCP) Exam questions and answers latest 2023Predominant form of DNA
Right-handed B
DNA and histone interaction is due to what type of bond?
Ionic
What stains to the minor groove of DNA duplex?
SYBR Green
What is the most abundant type of RNA?
Ribosomal RNA (rRNA)
...
MB (ASCP) Exam questions and
answers latest 2023
Predominant form of DNA - correct answer Right-handed B
DNA and histone interaction is due to what type of bond? - correct answer Ionic
What stains to the minor groove of DNA duplex? - correct answer SYBR Green
What is the most abundant type of RNA? - correct answer Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - correct answer Genome and mRNA
What increases half-life of mRNA? - correct answer 5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - correct answer
Euchromatin
What is the most common type of eukaryotic DNA? - correct answer Satellite DNA
What specimen has the most DNA according to molecular weight? - correct answer
White Blood Cells
What amino acids compose histones? - correct answer Lysine and Arginine
Amino acid that all proteins start with: - correct answer Methionine
What protects the DNA strand during replication? - correct answer Single-stranded
binding protein
Leading Strand - correct answer 3' to 5' template
Lagging Strand - correct answer 5' to 3' template
What DNA strand is being copied in transcription? - correct answer Antisense Strand
First step after newly formed mRNA is made? - correct answer Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? -
correct answer Telomerase
What polymerase has repair activity? - correct answer Beta/Epsilon
, What polymerase acts on leading strand? - correct answer Delta
What polymerase acts on lagging strand? - correct answer Alpha
Which polymerase is used for mitochondrial replication and repair? - correct answer
Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - correct answer Topoisomerase
Which RNA polymerase is mainly for transcription? - correct answer RNA Pol II
Which polymerase is involved with mRNA synthesis? - correct answer RNA Pol II
What inactivates RNAses? - correct answer Diethyl pyrocarbonate (DEPC) or bake
glassware for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? - correct answer
Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity? - correct answer Restriction
Enzyme Type II
What is necessary to make cDNA? - correct answer Reverse transcriptase
Number of SNPs in a human genome? - correct answer 11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? - correct answer 3
billion base pairs
The polymerization of acrylamide requires: - correct answer Catalyst (APS or TEMED)
or Light activation
How does agarose gel pore size relate to concentration? - correct answer Inversely
proportional
What is denatured during a southern blot? - correct answer Hydrogen bonds (inter-
strand bonds)
Melting temperature formula - correct answer Tm = (4C x GC Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe? - correct answer 5' G
Proper GC content of primer - correct answer 35-65%
Inhibitors of PCR - correct answer Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and
Bromophenol Blue
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