Enzymes Lab Report
Note: Parts that are filled out are in yellow for viewing convince
Instructions: In this lesson, we briefly discussed how cyclin-kinase complexes can malfunction due to mutations to
their genetic coding. In this lab activity, you will investigate the direct effects of environm...
enzymes lab report note parts that are filled out are in yellow for viewing convince instructions in this lesson
we briefly discussed how cyclin kinase complexes can malfunction
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Enzymes Lab Report
Note: Parts that are filled out are in yellow for viewing convince
Instructions: In this lesson, we briefly discussed how cyclin-kinase complexes can malfunction due to mutations to
their genetic coding. In this lab activity, you will investigate the direct effects of environmental factors on enzymes.
How could things like fever, hyperthermia, changes in blood pH, or the concentration of the enzyme affect how these
protein complexes function in the molecular control system of cell division? You will select two of these three factors
to test during your experiment. Submit your lab report to your instructor when completed.
Title: How Factors affect Enzyme CatalystHow Factors Affect Enzyme Catalyst
Objective(s): The objective in Part A is to test the effect of the concentration on the
reactivity with H2O2 to see if the reaction amount or speed changes. The objective in Part
C is to test the effect of pH on catalase with H2O2 to see if the reaction amount or speed
changes.
The objective of part A is to test the effects of concentration on the reactivity with H202 to see if the
reaction speed or amount changes. The objective in Part C is to test the effect of pH on catalyst with
H202 to see if the reaction speed or amount changes.
Hypothesis: (Part A) If the concentration increases then the reactivity will increase as well
as the speed. (Part C) If the pH level increases then the reactivity and speed will increase
too.
Part A: if the concentration increases than the reactivity and speed will increase.
Part B: if the pH level increases than the reactivity will increase as well as the speed.
As described above, you will be testing the effects of temperature, concentration, or pH on
enzyme activity. Select two of these factors for your investigation and create a hypothesis for
each. Remember, each part of your experiment will have a different independent variable.
Review the procedures section. Then write a prediction for each part of your investigation. Be
sure to compare your independent variable to your dependent variable using an "if /then” format
for each hypothesis statement.
Lab Safety:
Cover your work surface with newspaper or other covering to protect from spills.
Protect your eyes using goggles or protective glasses
If sodium bicarbonate or vinegar solution gets in the eye or on the skin, flush with water.
Oven mitt or glove for handling hot liquids should be used.
This study source was downloaded by 100000858936669 from CourseHero.com on 01-21-2023 11:18:00 GMT -06:00
A list of materials and summary of steps is provided for you. You should select the procedures
that match the factor you are testing. You only need to test two factors, not all three.
Materials:
Pick one of the following: potatoes, kiwi, peaches, cherries, apricots, bananas,
watermelon, or pineapple
Baking soda (if testing pH)
Water (and a way to warm it if testing temperature)
Ice cubes (if testing temperature)
Vinegar (if testing pH)
Hydrogen peroxide (can be purchased at a grocery store)
Glasses, cups, small bowls, or jars
Something to crush fruit or veggie
Large containers to hold hot and cold water (if testing temperature)
Measuring cups
Marker, paper, and tape for labeling
Timer
Summary of Steps:
Procedures for All Tests:
1. Catalase breaks down hydrogen peroxide into water and oxygen. The oxygen will
appear as bubbles in solution. For each test, allow the reaction to complete in ALL
test tubes before recording results.
2. Rank the test tubes in by the reactivity scale below and record your results in the data
section.
This study source was downloaded by 100000858936669 from CourseHero.com on 01-21-2023 11:18:00 GMT -06:00
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