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C785 Biochemistry preassessment with complete solution
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C785 Biochemistry preassessment with complete solution
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C785 Biochemistry preassessment1. Assuming 100% reaction efficiency, how many DNA copies are
created after the completion of four complete PCR cycles?: Answer- 16
2. What is the function of DNA polymerase in the process of PCR?:
Answer- It recog- nizes the primers and uses the available dNTPs to
replicate the template DNA sequence.
3. What are possible blood types of the parents of a patient with blood
type AB?: Answer- A and B
4. 115 116 117 118
Amino Acid Number Tyr Gly Thr Pro
Amino Acid Sequence TAC GGG ACA
CCA DNA Sequence
What is the amino acid in position 118 that results from a single
nucleotide insertion immediately following the three guanines that encode
for glycine 116?: Answer- Thr
5. In sickle-cell disease, the glutamic acid (Glu) at position number seven
is mutated to valine (Val).
What change in codons corresponds to this mutation?: Answer- GAA to GTA
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, 6. Which type of mammalian DNA damage repair requires the presence of
the chromosome that is homologous to the damaged chromosome?:
Answer- Recombi- nation
7. Xeroderma pigmentosum (XP) is a recessive genetic disease that
occurs when one or more of the genes that perform nucleotide excision
repair are nonfunctional.
Why do XP patients have a much higher incidence of skin cancer than the
general population?: The mutation rate of all other genes is higher due
to failure to repair.
8. Which mRNA sequence results in a polypeptide that contains three
amino acids?: 5' - CUU GAU ACU UAG - 3'
9. Which template strand DNA sequence would result in the amino
acid (Ser)?: 5' - CGA - 3'
10. What is the complementary sequence for the following DNA sequence?
5' - GCC ATC TCG AAT - 3': 5' - ATT CGA GAT GGC - 3'
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