Portage Microbiology Lab Exam 3 Latest 2022 List the four main steps used to prepare a DRY mount, and indicate which step is optional. 1 - Clean slide2 - Circle area on slide for specimen placement (OPTIONAL)3 - Apply organism to slide4 - Air dry at room temperature
Why is it important to first cl...
Portage Microbiology Lab Exam 3 Latest 2022 List the four main steps used to prepare a DRY mount, and indicate which step is optional. ✔✔1 - Clean slide2 - Circle area on slide for specimen p lacement (OPTIONAL)3 - Apply organism to slide4 - Air dry at room temperature Why is it important to first clean your slide before applying your sample? ✔✔You must first remove any unwanted contaminants from the slide otherwise it may be difficult to dist inguish between the pathogen of interest and a contaminant. You wish to study the motility of a particular bacterium. What type of mounting technique would you use? Explain your answer. ✔✔A wet mount technique is the best technique to use when studying mo tility of an organism because the sample remains viable (heat fixing kills the sample). What dye do Gram -positive bacteria primarily retain? ✔✔crystal violet Why are Gram -positive bacteria able to retain the crystal violet dye? ✔✔They contain a thick peptidoglycan layer in their cell wall that readily retains the dye. Identify the Gram status (positive or negative) and shape of the bacteria pictured below. ✔✔Gram -
negative; Bacillus (rod) When might you want to utilize a negative stain technique in the l ab? What is the name of the primary dye used in this technique? ✔✔A negative stain is used to identify organisms with an opaque structure. Nigrosin dye.
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