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STRAIGHTERLINE BIO250L Lab 6 Food Microbiology 2022

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1. Escherichia coli is one of the most common causes of foodborne illness, yet it is also a common microorganism found within the human gut. What explains this disparity? Harmful E. coli that are in polluted food and water are a straine of E.coli called Enterohemorrhagic strains. Harmless strain...

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  • April 14, 2022
  • 7
  • 2021/2022
  • Exam (elaborations)
  • Questions & answers

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  • straighterline bio250l lab 6 food microbiology 2022

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  • BIO 250L
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By: tanyajoyce • 1 year ago

Incomplete and done very poorly. The labs aren't hard. Do them yourself and you will have better answers.

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Lab 6 Food Microbiology BIO250L

Student Name:
Access Code (located on the underside of the lid of your lab kit): Click here to enter text.


Pre-Lab Questions
1. Escherichia coli is one of the most common causes of foodborne illness, yet it is also a common

microorganism found within the human gut. What explains this disparity?

Harmful E. coli that are in polluted food and water are a straine of E.coli called

Enterohemorrhagic strains. Harmless strains are part of the normal microflora of

the gut. These benefit the host by producing vitamin K. Without these harmless

strains we would not be able to produce vitamin Kand would have to depend on

supplements.



2. There are several foods and beverages listed in this lab that are created through the actions of

microorganisms. Name five of the foods and the specific microorganisms that are used to make

each of them.

Yogurt- Lactobacillus, Milk- Listeris monocytogenes, Cheese- Lactococcus,

Lactobacillus, and Streptococcus families, Breead- Saccharomyces cerevisiae,

Frute- E.coil.



3. Using an online research tool such as the A-Z index on the CDC website, explain why Listeria

monocytogenes is a major threat to pregnant women and their developing fetuses, who are ten

times more likely to get sick than other demographic groups.

Pregnant. Women are 10 timmes more likely to catch Listeria monocytogenes

than other people. Listeria monocytogenes can cause

, Lab 6 Food Microbiology BIO250L

miscarriage, still birth, and pre-term labor in pregnant women. In newborn’sit can

cause serious illness and death.



EXPERIMENT 1: Assessing the Bacterial Load of Milk with Methylene Blue
Results Tables
Table 1: Time Required for Methylene Blue Color Change

Start Time/Date End Time/Date Time Elapsed
Milk Sample
(Step 10) (Step 11) (End Time-Start Time)
0 hours 2:00 PM 3/8/19 5:30 PM 3/8/19 3.5 Hours
1 hour 2:01 PM 3/8/19 4:20 PM 3/8/19 2 Hours 19 Minutes
3 hours 2:02 PM 3/8/19 3:30 PM 3/8/19 1 Hour 28 Minutes
4 hours 2:03 PM 3/8/19 2:55 PMM 3/8/19 52 Minutes


Post-Lab Questions
1. On average, which sample took the least time to become white? Why do you think this was the

case?

The 4- hour sample took the least amount of time to become white. We think it

did because the milk had less oxygen in it since it had been sittingout.



2. On average, which sample took the most time to become white? Why do you think this was the

case?

The 0- hour sample took the most time to become white. We think it didbecause it

had more oxygen since it hadn’t been sitting out very long.

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