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Exam (elaborations) PHYS 101 (PHYS101 Student Exploration: Torque and Moment of Inertia Gizmo; 2021 $7.49   Add to cart
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Exam (elaborations) PHYS 101 (PHYS101 Student Exploration: Torque and Moment of Inertia Gizmo; 2021
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  • PHYS 101
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  • University Of Illinois - Urbana-Champaign

Exam (elaborations) PHYS 101 (PHYS101 Student Exploration: Torque and Moment of Inertia Gizmo; 2021

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  • April 13, 2022
  • 4
  • 2021/2022
  • Exam (elaborations)
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  • exam elaborations phys 101 phys101 student exploration torque and moment of inertia gizmo 2021

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  • University Of Illinois - Urbana-Champaign
  • PHYS 101
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Lab 8 Notebook

BS08 Biochemical Assays for Bacterial Antigen Detection

Objective: To understand the assays available for detecting bacterial or viral antigens.

Antigen – antibody generating pathogen

Notes:

 ELISA: Enzyme-Linked Immunosorbent Assay utilizes antibodies and colorimetric readout
to indicate and antigen of interest.
o Sandwich ELISA





Each well is labeled with an antibody (A)

 Antibody= blue y-shaped structure, adhere to the bottom of the
well
 To each well, you would then add a patient sample (i.e., serum or cultures
that you have grown)
 If antigen is present and recognized by the antibody, it would be bound
shown in B.
 Arrows indicate wash steps
 Once antigen is bound, you wash to remove and antigen that is
unbound.
 Part C: capture of the antigen to the antibody
 Incubation with a secondary antigen that also recognizes the antigen.
 Reason why it’s called a sandwich, because your antigen is now
sandwiched between 2 different antibodies.
 Secondary antibody has a tag on it (D)
 Tag either a enzyme or a substrate that will react once activated.
 Wash performed to the wash away the unbound secondary antibody.
 Well E: final step where everything is activated, and you get a colorimetric
change.
 Well E can have a very dark color or a light-yellow color, or some
won’t change at all
 From A to D = most likely to be a clear culture.
o Notes: (shown is a 96 well ELISA)
 Color intensity = abundance of antigen


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