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chem 120 week 7 :OL Lab 13- Protein Synthesis Updated
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CHEM120OL, Week 7 Lab
OL Lab 13: Protein Synthesis
Learning Objectives
• Explain the translation process from mRNA to amino acid
• Explain post-translational modification of proteins
• Explain protein synthesis processing in the ribosome
• Explain the primary, secondary, tertiary and quaternary structures of
protein
• Explain the basic principles of mass spectrometry (MALDI-TOF)
Introduction
In the Protein Synthesis lab, you will learn about the difference between
protein synthesis in prokaryote (using E. coli) and eukaryote (using Chinese
hamster ovarian (CHO) cells).
Prepare recombinant Erythropoietin and use the mass spectrometer
Your first task in the lab will be to prepare recombinant erythropoietin (EPO)
that is transfected into E. coli and CHO cells. The lab assistant will prepare
the recombinant EPO and you will measure the mass to charge ratio using a
mass spectrometer. Not sure how to handle the mass spectrometer? No
worries! You can just take out your labpad and find an animated video to
learn the basics.
Study the translation process from mRNA to amino acids
You will also learn about the translation process from mRNA to amino
acids and how amino acids are assembled to proteins. A 3D animation will
describe how codons are translated into amino acids, how these amino
acids are joined together by peptide bonds creating a primary structure of
protein, and how the primary structure is folded into secondary, tertiary
and quaternary structure.
Investigate doping in bike athletes
In the last part of the Protein Synthesis lab, you will use mass
spectrometry to investigate if any athletes are using recombinant human
erythropoietin (rhEPO) as a doping substance. You will do so by
collaborating with the doping agent who collects urine samples at a large
bicycle race.
Will you be able to detect if any of the athletes are using doping?
Part 1: Complete Labster Lab: Protein
Synthesis Part 2: Report and Reflection
Purpose: Describe in complete sentences and in your own words, the purpose
of this experiment.
To teach us about the process of protein synthesis as well as doping in
athletes.
, CHEM120
OL, Week 7 Lab
Observations: Record three observations from the simulation.
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