Exam (elaborations)
BIOD 171 LABORATORY EXAMS Q & A
- Course
- Institution
BIOD 171 LABORATORY EXAMS Q & A BIOD 171 LABORATORY EXAMS Q & A
[Show more]
Seller
Follow
ProfessorBanks
Content preview
BIOD 171: LABORATORY EXAMS Q & ALAB VIII
1. The word ‘antigen’ is actually a combination for what two words?
Antibody-generating
2. For the ELISA assay depicted below, what do the arrows indicate?
The arrows indicate the wash steps.
3. What would happen in terms of the colorimetric readout if you forgot to
do the wash step after Step D (above)?
, BIOD 171: LABORATORY EXAMS Q & A
All of the wells would have a dark color. The intensity of the readout is dependent
upon the amount of the labeled secondary antibody present in the well. Since the
unbound secondary antibody was not washed away, all wells would react equally.
1. In a Western blot are separated based on its size.
proteins
2. When setting up a western blot, what is the purpose of the blocking step
post-transfer?
Blocking prevents non-specific binding of the antibody—you only want the
antibody to bind to its specific target.
3. When developing a western blot what two factors influence the intensity of
the band?
Both the amount of protein loaded as well as the duration (time) of development is
directly proportional to band intensity.
4. Identify (specific name) given to the region of the western blot indicated by
the arrow.
The arrow is pointing to the dye front.
, BIOD 171: LABORATORY EXAMS Q & A
1. What is the common name for agglutination?
Clumping
, BIOD 171: LABORATORY EXAMS Q & A
2. In the wet lab portion of the video, what pathogen was the
agglutination assay specifically designed to test for? Choose all that
apply.
A. E coli
B. Salmonella
C. Staph
D. Strep
C. Staph
3. What was the purpose of the first quadrant of the agglutination
assay containing only the reagent?
The first quadrant served as the reagent only negative control—you want to
ensure the reagent by itself does not clump but rather requires the presence of
Staph.
4. Based on the image below, which row (A or B) is negative for agglutination?
Row A is negative for agglutination.
5. A patient with type O blood is sent to the lab for a blood-clotting
assessment. Since type O blood contains no antigens, which quadrant (#1-
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller ProfessorBanks. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $17.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
79373 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now