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BIOS 242 Week 2 iLab: Aseptic Technique

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BIOS 242 Week 2 iLab: Aseptic Technique

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  • February 21, 2022
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  • 2021/2022
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BIOS 242 Week 2 iLab: Aseptic Technique



ASEPTIC TECHNIQUE

Introduction

The purpose of this lab is to culture cells successfully without contamination using

aseptic technique. If contamination occurs, the lab experiment will be inaccurate.

Biological contamination can happen via dirty supplies, airborne toxins, and unkempt

workstations. This aseptic technique lab is designed to guide you through a successful

(uncontaminated) lab experiment. With these aseptic techniques it will help to yield a

level of protection for the cell culture. Aseptic techniques require sterile media, sterile

agents, proper hygiene, and a clean workspace.



Findings: Mannitol Fermentation test 100% positive.



Procedure



Conduct Contamination Test:



1. Click the New Unknown button. A window will open asking you to enter a

label and select a subgroup.

2. Type Technique1 in the “Enter a Label” line.

3. From the Subgroup dropdown menu, select Asceptic Technique. DO NOT

CLICK Auto-inoculation allowed. Click OK.

, 2


4. Record details of your case study scenario.

5. Record the Gram Reaction by clicking the appropriate radio button. Click

Record Gram Reaction.

6. You will need to indicate the results of the Gram Stain in your Lab Report.

7. From the Media dropdown menu, select Phenol Red Glucose Broth Durham

Tube. The Media dropdown menu is to the far right of the New Unknown

button.

8. Enter PhenolRedGlucose in the Medium Label window to label your sample.

Click OK. Two tubes will appear on your workbench. Take a careful look at

the sample tube (the one on the right.) Is there media in the Durham Tube

(this is the tube that is upside down in the medium.)

9. Note the status of the Traffic Lights for Inoculation and Contamination in the

upper right hand corner of the lab software window.

10. Right click on the phenol red tube (the tube on the right) and select Remove

caps/lids.

iLab: Aseptic Technique and Bacterial Anatomy and Morphology Page 4

11. Look at the Inoculation/Contamination traffic lights. What color

are these lights?

12. Right click on the phenol red tube and select Replace caps/lids. Look

at the Inoculation/Contamination traffic lights. How has the color

of each light changed? Record these changes for your lab report.

13. Using your cursor, drag the phenol red tube and place it on the 37

degree incubator (this is the incubator on the right side of the

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