BIOLOGY 171 BIO 171 / BIO171 MICRO BIO LAB EXAMS #1 - 6
7 views 0 purchase
Course
BIOD 171/BIOD171
Institution
Portage Learning
PORTAGE LEARNING
BIOLOGY 171
BIO 171 / BIO171
MICRO BIO
LAB EXAMS #1 - 6
1. What three elements are used in an autoclave to sterilize equipment? heat, pressure, and steam
2. What is the minimum temperature an autoclave must be set at to achieve sterile condition? 125°C
3. If you are ...
portage learning biology 171 bio 171 bio171 micro bio lab exams 1 6
Written for
Portage Learning
BIOD 171/BIOD171
All documents for this subject (14)
Seller
Follow
Marilyn77
Reviews received
Content preview
PORTAGE LEARNING
BIOLOGY 171
BIO 171 / BIO171
MICRO BIO
LAB EXAMS #1 - 6
1. What three elements are used in an autoclave to sterilize equipment? heat, pressure, and steam
2. What is the minimum temperature an autoclave must be set at to achieve sterile condition? 125°C
3. If you are working in a lab in which an autoclave is not available, and you are pressed for time,
which would you chose to best sterilize your equipment? Hot steam or hot air? Explain why you
chose your answer. Hot steam is the best choice as you can achieve a sterile environment in
a matter of minutes whereas hot air will take several hours to achieve the same effect.
4. What type of incubator is pictured below? Fixed incubator
5. At what temperature is the fixed incubator set to, as presented in the lab video? 37°C
6. At what temperature should you refrigerate bacterial samples? Explain why this is beneficial. 4°C.
This temperature slows bacterial growth and prolongs the life of the sample.
7. What are the FOUR types of gloves presented in the lab video? Latex, Nitrile, Thermal cold,
Thermal heat
8. What THREE rules were discussed in regards to lab safety that would protect you and others
from contamination? 1. Never eat or drink in the lab
2. Always wear appropriate PPE
3. Never leave the lab wearing PPE
9. What are the main sections that should be found in a lab notebook? Name at least 4. Objective,
Procedure, Notes, Results and Deviations
LAB 2
4. How should you hold the microscope when you move it or carry it? You should hold it with
one hand on the neck and one hand under the base
5. List the four magnification strengths found on the objectives of the light microscope shown in
the lab video. 4x, 10x, 40x, 100x
, 6. As you looking through the microscope you notice your sample is slightly off-center—what
component of the microscope would be used to bring the sample into the center of the image?
Stage adjustment knobs
7. What objective power is best suited if you are uncertain what the sample is and where to begin?
4x (or lowest power objective)
8. You are viewing a sample of bacteria that is 3 mm in diameter through a 40x objective lens. The
eyepiece has a magnification power of 10x. What size will the sample appear through the
eyepiece? 1200 mm in diameter (or 400x’s larger)
9. Based on the microscope shown in the lab lecture, which objectives would NOT require placing
oil on the slide? 4x, 10x and 40xResults: Magnification = objective x eyepiece
LAB 3 EXAM
1. List the 4 main steps used to prepare a WET mount and indicate which (if any) step is optional.
1 – Clean slide
2 – Circle area on slide with wax/hydrophobic
pen 3 – Apply organism to slide
4 – View under microscope
*Note: there are no optional steps in the wet mount.
2. When preparing a glass slide for a dry mount, what cleaning solution was used? 70% ethanol
3. You wish to study the motility of a particular bacterium. What type of mounting technique
would you use? Explain your answer. A wet mount technique is the best technique to use
when studying motility of an organism because the sample remains viable (heat fixing
kills the sample).
4. What dye do Gram-negative bacteria primarily retain? Safranin
5. Why are Gram-positive bacteria able to retain the crystal violet dye? They contain a thick
peptidoglycan layer in their cell wall that readily retains the dye.
6. Identify the Gram status (positive or negative) and shape of the bacteria pictured below. Gram-
Negative; Bacillus (rod)
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Marilyn77. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $16.99. You're not tied to anything after your purchase.
