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B101 Week 8 Experiment Answer Sheet
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WEEK 8 EXPERIMENT ANSWER SHEETPlease submit to the Week 8 Experiment dropbox no later than Sunday midnight.
SUMMARY OF ACTIVITIES FOR WEEK 8 EXPERIMENT ASSIGNMENT
Experiment 8 Exercise 1 – Species Interactions: Competition
Experiment 8 Exercise 2 – Biomes (Part I and II)
Experiment 8 Exercise 1: Species Interactions: Competition
In this exercise you will be evaluating the effect of competition on the population size of two species
of microorganisms. Be sure you have read through the readings for Week 8 as well as the
introductory information for the Week 8 Experiment. When you are ready to begin, open in the
following website:
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Glencoe-McGraw Hill. No date. Population Biology
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http://glencoe.mcgraw-hill.com/sites/dl/free/0078759864/383928/BL_04.html
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Procedure
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A. Click on the Information button on the bottom and read through the material before
beginning. You will need to scroll down to read all of the information. Close the window when
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you are done. Note that the two species we will be using will be competing for the same food
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source; bacteria.
B. First, you need to set up the experiment by distributing the two species to the three test
tubes.
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a. Click on the pipette (the purple bulb) in the flask containing P. caudatum, fill it and
place the contents in Tube #1.
b. Then click on the pipette in the flask containing P. aurelia, fill it and place the contents
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in Tube #2.
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c. Finish by putting a pipette full of both species in the Tube #3.
C. Answer the question below before proceeding.
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Question
1. The number of P. caudatum and P. aurelia grown alone would be expected to increase until
the population size reaches the carrying capacity of the test tube. What do you think will
Updated October 2013
This study source was downloaded by 100000805705997 from CourseHero.com on 11-03-2021 21:23:33 GMT -05:00
https://www.coursehero.com/file/39455795/Week-8-Experiment-DONEdoc/
, happen in terms of population growth in Tube #3 that contains both species combined and
why (2 pts)?
I really think that it isn’t going to do anything, and it will cancel each other out to the point where
it is kind of a stalemate.
Procedure (continued)
D. You now need to count the number of organisms in each Tube beginning on Day 0 and
continuing every 2 days until you reach Day 16. These values will need to be recorded in Table
1 below (do NOT use the Table provided by the website).
a. Click on the Microscope to get started.
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b. Click on Clean microscope slides and then Take Sample.
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c. Click on the first slide and drag it on to the microscope. Count the number of P.
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caudatum (note its shape) and multiply by 2 to get the number of cells per ml (your
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slide holds 0.5 ml). Record this number in the Table below; this is Day 0.
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d. Next, click on the second slide and drag it to the microscope. Count the number of P.
aurelia (note its shape), multiply by 2 and record this number in the Table for Day 0.
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e. Finally, drag the third slide on to the microscope and count the number of each type of
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organism, multiply by 2 and enter the data into the Table.
f. Click on Clear Slides (on the bottom) and then on the Calendar that says Day 0 to
advance it two days.
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g. Repeat steps b - f until you reach 16 days.
h. As the days go on, you will have more and more individuals to count. Click on the Grid
On button on the microscope to make them easier to count.
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Table 1. Results (4 pts).
Grown Separately (cells per ml) Grown Together (cells/ml)
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Day P. caudatum P. aurelia P. caudatum P. aurelia
0 2 2 2 2
2 10 10 12 8
4 26 50 22 34
Updated October 2013
This study source was downloaded by 100000805705997 from CourseHero.com on 11-03-2021 21:23:33 GMT -05:00
https://www.coursehero.com/file/39455795/Week-8-Experiment-DONEdoc/
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