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BIOD 171 Lab 9 - notebook Unknown pathogen analysis

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BIOD 171 Lab 9 - notebook Unknown pathogen analysis/BIOD 171 Lab 9 - notebook Unknown pathogen analysis/BIOD 171 Lab 9 - notebook Unknown pathogen analysis/BIOD 171 Lab 9 - notebook Unknown pathogen analysis/BIOD 171 Lab 9 - notebook Unknown pathogen analysis/BIOD 171 Lab 9 - notebook Unknown patho...

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Lab 9 Notebook Title: SK09 Unknown Pathogen Analysis
Objective: To identify an unknown pathogen(s) based on the given assays developed during this course
Procedure:
Notes:
Two samples from the hospital were brought to your lab for diagnosis. After culturing the
samples on LB agar plates another co-worker began the analysis. However, due to an emergency
your co-worker has left the remaining work to you. Before leaving your co-worker informs you of the 2 liquid culture samples we accidentally combined before the Gram stain was started.
However, the 2 cultures remain separated on LB plates for future tests. Goal: To correctly identify the two pathogens
Results:
-Gram Stain
-Purple cocci clusters which are gram positive
-Pink rods which are gram negative
-MacConkey Plates (grows only gram negative)
-Culture A: no growth observed
-Culture B: gram negative
-lactose fermentation (E. coli)

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