Additive fixative - Study guides, Class notes & Summaries

An example of an additive fixative is one that contains: - Answer picric acid Microscopic examination of an H&E stained section fixed in formalin shows marked nuclear bubbling. One most often sees this artifact if the specimen is processed following: - Answer incomplete fixation Microscopic evalution of H&E stained sections from a surgically removed small bowl specimen shows an absence of much of the epithelium in otherwise normal tissue. This most likely resulted from: - Answer d...

Carsons Mordant . substance’s or metals that act as a link between dye and tissue A mordant is a metal with a valency of at least two. The two commonest metals used in histotechnology are aluminum and ferric iron, both with valencies of three. The attachment of mordant to dyes is by means of a covalent and a coordinate bond. Fixative additive fixation- stabilizes proteins by denaturing proteins (chemical, heat) and combines with fixative molecule becoming insoluble non additive ...

Carsons Mordant . substance’s or metals that act as a link between dye and tissue A mordant is a metal with a valency of at least two. The two commonest metals used in histotechnology are aluminum and ferric iron, both with valencies of three. The attachment of mordant to dyes is by means of a covalent and a coordinate bond. Fixative additive fixation- stabilizes proteins by denaturing proteins (chemical, heat) and combines with fixative molecule becoming insoluble non additive ...

An example of an additive fixative is one that contains: CORRECT ANSWER-picric acid Microscopic examination of an H&E stained section fixed in formalin shows marked nuclear bubbling. One most often sees this artifact if the specimen is processed following: CORRECT ANSWER-incomplete fixation Microscopic evalution of H&E stained sections from a surgically removed small bowl specimen shows an absence of much of the epithelium in otherwise normal tissue. This most likely resulted from: CORRECT...

Carsons Histotechnology Question and answers already passed 2023/2024Mordant . substance’s or metals that act as a link between dye and tissue A mordant is a metal with a valency of at least two. The two commonest metals used in histotechnology are aluminum and ferric iron, both with valencies of three. The attachment of mordant to dyes is by means of a covalent and a coordinate bond. Fixative additive fixation- stabilizes proteins by denaturing proteins (chemical, heat) and combin...

Histotechnology - Tissue FixationAdditive fixatives Mercuric chloride Chromic acid Picric acid Formaldehyde Glutaraldehyde Osmium tetroxide Potassium dichromate Glyoxal Zinc salts Nonadditive fixatives Alcohols Acetone Acetic Acid Coagulant Fixatives Mercuric acid Chromic acid Picric acid Zinc salts Alcohols Acetone Acetic acid Noncoagulant Fixatives Formaldehyde Glutaraldehyde Osmium Tetroxide Potassium Dichromate Glyoxal Are coagulant fixatives addit...

BOC Study Guide Histotechnology 2nd ed.- Fixation (HT)An example of an additive fixative is one that contains: - correct answers picric acid Microscopic examination of an H&E stained section fixed in formalin shows marked nuclear bubbling. One most often sees this artifact if the specimen is processed following: - correct answers incomplete fixation Microscopic evalution of H&E stained sections from a surgically removed small bowl specimen shows an absence of much of the epithelium in othe...

An example of an additive is one that contains: A. Picric acid B. Acetic acid C. Ethyl alcohol D. Acetone - correct answer Picric Acid When compared with tissue fixed in formalin, tissue fixed in zinc-formalin will show: A. Better ultrastructural preservation B. Decreased immunoreactivity C. Increased enzyme activity D. Superior nuclear detail - correct answer Superior nuclear detail Micr...

Acetic Acid - Answer Non coagulant, penetrates quickly and leaves tissues very soft. Used often as an additive to precipitate and preserve the nucleoproteins and fix the nuclei. Acetic Acid - Answer Swells tissues dramatically by breaking collagen bonds and allowing bonds to absorb water. Used as additive to counter-act the shrinking effect of other fixatives. Formaldehyde - Answer Non coagulant and additive fixative that reacts with amino groups that link protein chains together....

Histotechnology- FixationFixation: - correct answers Alteration of biological tissue by chemical or physical means which stabilizes proteins found in tissue, preventing further metabolic activity. Purpose of Fixation: - correct answers To kill tissue and preserve it by limiting the effects of decay, putrefaction, and autolysis. Fixation also hardens tissue which makes it easier to gross, and easier to section once it is processed and embedded. Physical Agents: - correct answers - Heat and ...