Bch 403 - Study guides, Class notes & Summaries

The polypeptide shown below needs to be separated from other polypeptides of similar size (at pH 7.0). What purification technique is best suited to isolate this peptide? A. Nickel affinity chromatography, since it contains a histidine residue that can bind metal. B. Size exclusion chromatography, since the polypeptide has some hydrophobic residues. C. Anion exchange chromatography, since the polypeptide has a net positive charge. D. Anion exchange chromatography, since the polypeptide ha...

how are H bonds formed in beta sheets? Correct Answer-- between strands - H-bonds are lateral why do proteins fold if it would lower entropy? Correct Answer-- the hydrophobic effect - deltaH --> all of the interactions happening w/in the protein are favorable + overcome the unfavorable entropy of folding what types of interactions stabilize 3* structures? Correct Answer-- H-bonds - ion pair / salt bridges - van der Waals interactions: weak electrostatic interactions b/t induced dipo...

In E. coli, 5S rRNA is cleaved out of the pre-rRNA operon by: a. RNase III b. RNase E Correct Answer-RNase E The 5' cap: a. Does not play a role in translation b. Interacts with the poly A tail during transport out of the nucleus c. Protects the mRNA from 5' exonucleases d. Is acetylated e. Is sometimes found on E. coli mRNA Correct Answer-Protects the mRNA from 5' exonucleases The addition of the poly A tail: a. Occurs during the elongation phase of transcription b. Occurs during...

E1: Why do proteins fold? - Answer A and B are correct (It is entropically favorable for water & It is enthalpically favorable for the protein) E1: The structural changes in hemoglobin that contribute toward its positive cooperativity for oxygen consist of: - Answer All of the above ( movement of iron into the plane of the heme & movement of a neighboring veiling residue due to strain from changes in the heme & the breaking of a covalent bond with the regulatory molecule BPG

WATER Correct Answer-Many biochemical reactions that form biopolymers from monomeric units involve the removal of WATER Correct Answer-When monomers covalently attach to each other to form a macromolecule what product is often formed? CONFORMATION Correct Answer-Which of the following can be determine whether an enzyme is functional or not? HYDROGEN BONDS Correct Answer-Which of the following can be is an important noncovalent interaction in biochemistry? OXYGEN AND NITROGEN Correct ...

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