Molar absorptivity - Study guides, Class notes & Summaries

MMSC 407 Final Exam questions and answers well illustrated. light - correct energy source that can be characterized in terms of wavelength, amplitude and frequency -the visible spectrum wavelength - correct distance between waves amplitude - correct height of a wave frequency - correct many waves pass in one second (related to speed) high energy waves - correct frequency, very short and compact wavelengths -e.g. violet low energy waves - correct frequency, lon...

Which of the following is an example of semi-quantitative test result? - ️️3+ Which of the following signals is light based? - ️️scatter Which of the following under-estimates the concentration but not over-estimates? - ️️downward shift What is the purpose of the reagent blank in spectrophotometric analysis? - ️️to subtract the color contribution of the reagent Which of the following describes a specimen blank? - ️️subtract the light absorption of patient serum. ...

MMSC 407 Exam Questions with All Correct Answers What is the distance between the crests of two successive waves - Answer-Wavelength What is the height of each wave - Answer-Amplitude What is the number of waves which pass an observation point in one second - AnswerFrequency What is it called when the wave bounces back to the source - Answer-Reflected What is it called when the wave is absorbed by the material - Answer-Absorbed What is it called when the wave passes through the material...

MMSC407 Exam Questions with Correct Answers What is the distance between the crests of two successive waves - Answer-Wavelength What is the height of each wave - Answer-Amplitude What is the number of waves which pass an observation point in one second - AnswerFrequency What is it called when the wave bounces back to the source - Answer-Reflected What is it called when the wave is absorbed by the material - Answer-Absorbed What is it called when the wave passes through the material - An...

beer-lambert law - ACCURATE ANSWERS A = εdc A = absorbance ε = molar absorptivity (L mol-1 cm-1) d = distance the light travels through the solution (the cuvette) in cm c = concentration of the solution (mol/L) Beer's Law calibration curve: Use the following data to generate a calibration curve of Absorbance vs Concentration at λmax = 420.6 nm. concentration 1: 6e-5 M; absorbance 1: 1.330 concentration 2: 2e-5 M; absorbance 1: 0.451 concentration 1: 1e-5 M; absorbanc...

what wavelength of lights in the electromagnetic spectrum has the highest energy correct answers A 400 nm the emission of light caused by chemical reaction is correct answers A. chemiluminescence Beer's law expression correct answers D. A= abc calculate the concentration of NADH, given that the absorbance is 340 nm is 0.5 and the molar absorptivity constant is 6220 M cm. The cuvette has a path length of 1 cm. correct answers C. 0.8 uM which component of a single beam spectrophotomet...

What color and what type of reaction does Baeyer's test indicate when reacted with a Alkane? STAYs purple NEGATIVE reaction What color and what type of reaction does Baeyer's test indicate when reacted with a Alkene? TURNS red POSTIVE reaction Bromine in CCl4 with Alkane turns the red-orange (NEGATIVE) Bromine in CCl4 with Alkene decolorizes (POSITIVE) Density Mass/ Volumes Beer's law A=ɛbc A=Absorbance Absorbance=2-log(%Transmittance) ɛ= molar absorptivity b= ...

What is a photomultiplier? - A vacuum tube consisting of a photoemissive cathode, a series of intermediate electrodes called dynodes, and a collection anode. What does a single-beam spectrophotometer do? - A single-beam spectrophotometer employs one beam of radiation that irradiates one cell. What happens in a deuterium lamp? - The lamp energy from the power source produces an excited deuterium molecule that dissociates into two atoms in the ground state and a photon and the energies of th...

What is the Beer-Lambert Law? - Answer- the linear relationship between absorbance and concentration of an absorbing species. What is the Beer-Lambert Law equation? - Answer- A = ebc A: absorbance e: molar absorptivity c: concentration b: path length DNA and RNA are absorbed at: - Answer- 260nm Proteins are absorbed at: - Answer- 280nm What is the melting temperature equation? - Answer- Tm = (4 x # of GC pairs) + (2 x # of AT pairs) Dilutions equation - Answer- V1C1 = V2C2 ...

Calculate the absorbance A of solutions whose percent transmittance is measures as 5%, 10%, 50%, and 100%. - Answers-A=-log(T) 1.3, 1.0, .30, and 0 respectively Give the block diagram of a visible spectrophotometer, illustrating the major components of the instrument in the correct sequence. - Answers-[Light source] ---many lambdas---> [Monochromator] --- one lambda---> [Sample cell] ---I---> [Photodetector] ------> [Readout] It is important that Kf for FeSCN2+ has a large valu...