Column chromatography - Study guides, Class notes & Summaries

BTC exam questions & answers grade A+ 2024/2025 A researcher is working with a protein that contains four subunits of differing molecular weights. If the researcher perfoms native PAGE, how many distinct bands should he see on the gel? - ANSWERSA. four B. ONE C. Two D.Three _______Is not used to detect protiens in gel - ANSWERSA. Coomassie Brilliant Blue B. Silver Stain C. TRYPAN BLUE D. Negative Stain (copper) Which is the correct fluid path in Liquid Chromatography system (Bio...

AQA GCSE COMBINED SCIENCE: TRILOGY 8464/C/2H Chemistry Paper 2H Mark scheme June 2023. CH4 + 2 O2 → CO2 + 2 H2O 1 AO2 5.7.1.3 Question Answers Extra information Mark AO / Spec. Ref. 01.2 hydrocarbon molecules in petrol have fewer carbon atoms than those in diesel petrol has a lower boiling point (range) than diesel allow converse throughout allow petrol is more flammable than diesel allow petrol is less viscous than diesel 1 1 AO3 5.7.1.3 Question Answers Extra information M...

Albert's Chapter 8 Exam 115 Questions with Verified Answers WITTF: machine that sorts fluorescently labeled cells and nonlabeled cells into separate containers using a strong electric field - CORRECT ANSWER fluorescence-activated cell sorter How are antibodies used to fluorescently label cells? - CORRECT ANSWER fluorescent molecule attached to antibody, which binds to a very specific cell type How does a fluorescent-activated cell sorter work? - CORRECT ANSWER individual cells travel s...

Column Chromatography (CC)

in size exclusion chromatography, you would Expect a. colored mol to elute off column prior to clear b. sm mol to elute off column faster than lg c. sm mol to bind to the column when the buffer pH is low d. sm mol to elute off column slower than lg mol - D. sm mol to elute off column slower than lg mol elevated serum pro and decreased albumin are associated with: a, glomerulonephritis b. cirrhosis. c. starvation/anorexia d. monoclonal gammopathy - d. monoclonal gammopathy beers law sta...

basic units of life that compose all living things - Answer-Cells characteristics shared by all cells - Answer--Cells are composed of 4 major macromolecules -Genetic material is carried and stored in the form of DNA -DNA is transcribed into RNA and RNA is translated into proteins -Their appearance, behavior, and function is dictated by proteins -They reproduce on their own -Respond to stimuli -Adapt to their environment -Are highly organized -Maintain a constant internal environment Ho...

Extraction - correct answers Transfer of a solute from one phase to another Eluent - correct answers Fluid entering the column Partition Chromatography - correct answers Separation based on solute dissolving into stationary liquid Ion-Exchange Chromatography - correct answers Separation based on charge, stationary phase is made of either negatively or positively charged beads Size Exclusion Chromatography - correct answers Separation based on molecular size, larger molecules elute fi...

carrier gas - correct answers mobile phase gas in gas chromatography cold trapping - correct answers splitless gas chromatography injection technique in which solute is condensed far below its boiling point in narrow band at the start of the column electron capture detector - correct answers gas chromatography detector that is particularly sensitive to compounds with halogen atoms, nitro groups, and other groups with high electron affinity flame ionization detector - correct answers A g...

• Typical NMR chemical shift for methyl protons adjacent to a ketone 2.1-2.6 ppm • Level of solvent on a TLC plate Below the spots when first placed in the developing jar, and then it will eventually rise to the top past the spots Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More Column Chromatography Provides a method for the separation and isolation of individual components of a mixture •...

What is the maximum amount of time a specific solute band will spend in the mobile phase of a chromatography column? - correct answers tm Which of the following is NOT an advantage of the use of OPEN TUBULAR columns (capillaries) over packed columns? - correct answers A. smaller plate height (H) B. [[[[greater column capacity]]]] C. faster flow rates D. No A-term in the van Deemter equation E. higher separation efficiency Which mode of chromatography separates compounds based on th...