Southern blot - Study guides, Class notes & Summaries

MB - ASCP Review Exam With Correct Questions and Answers Updated 2024 A+ Graded 100%. Her2/neu exhibits an overexpression, what would this indicate? - ANSWER-More aggressive growth and metastasis of the tumor cells in human breast cancer. Predominant form of DNA - ANSWER-Right-handed B DNA and histone interaction is due to what type of bond? - ANSWER-Ionic What stains to the minor groove of DNA duplex? - ANSWER-SYBR Green What is the most abundant type of RNA? - ANSWER-Ribosomal RN...

E. coli RNA Polymerase II - Answer DNA template, product: mRNA RNA Polymerase I - Answer DNA template Location: Nucleous Products: 18s, 5.8s, 28s rRNA RNA Polymerase II - Answer DNA template Location: Nucleus Products: mRNA, snRNA RNA Polymerase III - Answer DNA template Location: Nucleus Product: tRNA, 5s rRNA, snRNA Mitochondrial RNA Polymerase - Answer DNA template, product: mRNA Mammalian DNA polymerase α - Answer DNA template, product: primers HCV RNA Polymerase - A...

What techniques are used in molecular diagnosis? Correct Answer-Southern blot Dot blot/Reverse dot blot ***Polymerase chain reaction*** RT-PCR Mutation scanning: SSCP, DGGE, dHPLC Capillary electrophoresis Protein truncation test ***DNA sequencing*** Real-time PCR Invader assay In situ hybridization Microarrays What are the areas of application of molecular diagnostics? Correct Answer-Infectious Disease Neoplastic Disease ***Genetic Disease*** ***Identity Testing - fingerprint...

BCHM 270 EXAM QUESTIONS WITH CORRECT ANSWERS A physician would like to evaluate the elevated amounts of a gene being expressed in two different types of tumor cells in order to develop a personalized medicine. Which of the following techniques would be the most appropriate for this purpose? A. Western Blot B. Southern Blot C. Northern Blot D. Eastern Blot E. enzymatic assay - Answer-C A restriction enzyme can be used for which techniques? A. Southern blots and cloning. B. western blo...

What modification neutralizes the charges on histones and looses up the interactions between histones and DNA? - ANSWER ACETYLATION Two types of post-transcriptional modifications that take place in the mRNA of eukaryotes - ANSWER the addition of a CAP at the 5' end of the transcript and the addition of a POLY A sequence at the 3' end of the message Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally - ANSWER Hybridizes filter-bound DNA wi...

MCAT QuestionsWhich ideal solution exhibits the greatest osmotic pressure? a. 0.1 M MgCl2 b. 0.2 M NaCl c. 0.2 M CaCl2 d. 0.5 M Glucose C because the solution listed has the greatest concentration of solute particles. CaCl2 dissociates into three ions (van Hoff factor of 3), making the solution listed 0.6 M in solute particles. Glucose has an i of 1. Which event is directly mediated by a ligand-gated ion channel? a. Release of Ca2+ from the sarcoplasmic reticulum of a muscle fiber to ...

What are the steps of a southern blot? - ️️1. DNA is isolated and cut with restriction enzymes 2. Fragments are separated by gel electrophoresis 3. DNA fragments are exposed to a labeled probe (complementary DNA or RNA), complementary to region of interest What is RFLP? - ️️- Restriction Fragment Length Polymorphism - The number and location of restriction sites for a given restriction enzymes are not the same in all individuals What is the mapping difference between linear DNA f...

MCAT QuestionsWhich ideal solution exhibits the greatest osmotic pressure? a. 0.1 M MgCl2 b. 0.2 M NaCl c. 0.2 M CaCl2 d. 0.5 M Glucose C because the solution listed has the greatest concentration of solute particles. CaCl2 dissociates into three ions (van Hoff factor of 3), making the solution listed 0.6 M in solute particles. Glucose has an i of 1. Which event is directly mediated by a ligand-gated ion channel? a. Release of Ca2+ from the sarcoplasmic reticulum of a muscle fiber to ...

What modification neutralizes the charges on histones and looses up the interactions between histones and DNA? - Answer- ACETYLATION Two types of post-transcriptional modifications that take place in the mRNA of eukaryotes - Answer- the addition of a CAP at the 5' end of the transcript and the addition of a POLY A sequence at the 3' end of the message Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally - Answer- Hybridizes filter-bound DNA...

BCH 5413 EXAM ONE QUESTIONS WITH VERIFIED ANSWERS If you want to identify the protein PTEN on a Western blot, what is secondary antibody? - Answer-Fab can bind to primary antibody and Fc portion can bind to a HRP antibody so when bound to TMB will light up Which of the following increases the stringency of Southern Blot? Why? - Answer-Increased hybridization, more Interactions between target and probe PCR: primer - Answer-Short ssDNA sequence used in PCR, pair of primers used to hybridize...