Sodium dodecyl sulfate - Study guides, Class notes & Summaries

ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2024. Henderson-Hasselbach Equation - CORRECT ANSWER - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - CORRECT ANSWER - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the Nterminus. Salting Out (Purification) - CORRECT ANSWER - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. ...

messenger RNA (mRNA) is synthesized in what direction and is complementary and antiparallel to which DNA strand (coding or template)? correct answers synthesized in the 5'-->3' direction and is complementary and antiparallel to the DNA template strand. It is identical to the DNA coding strand. How are the basal layer of the epidermis and the innermost lining of the small intestine similar? A. both are nondividing tissues B. both are derived from ectoderm C. both are composed of squamo...

Henderson-Hasselbach Equation Ans- pH = pKa + log ([A-] / [HA]) Salting Out (Purification) Ans- Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography Ans- Separates sample based on size with smaller molecules eluting later. Ion-Exchange Chromatography Ans- Separates sample based on charge. CM attracts +, DEAE attracts -. May have repulsion effect on like charges. Salt or acid ...

Henderson-Hasselbach Equation Ans- pH = pKa + log ([A-] / [HA]) Salting Out (Purification) Ans- Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography Ans- Separates sample based on size with smaller molecules eluting later. Ion-Exchange Chromatography Ans- Separates sample based on charge. CM attracts +, DEAE attracts -. May have repulsion effect on like charges. Salt or acid ...

ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2023/2024 COMPLETE SOLUTIONS LATEST 2023/2024 Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Use d in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solu...

Henderson-Hasselbach Equation - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - Separates sample based on size with smaller molecules eluting later. Ion-Exchange...

Chapter 5: Proteins: Primary Structure Matching A) electrophoresis B) hydrophobic interaction C) enzyme-linked immunosorbent assay D) three-dimensional shape E) N-terminal amino acid F) negative charge G) nucleases H) chromophore I) foaming J) high level expression K) 2-mercaptoethanol L) positive charge M) cation exchange N) pI O) chymotrypsin P) C-terminal amino acid Q Sodium dodecyl sulfate 1. One of the reasons the primary structure is important for a protein is that it de...

SDS-Page abbreviated for Correct Answer-Sodium Dodecyl sulfate Polyacylamide Gel Electrophoretic SDS in the presence of protein and heat Correct Answer-wraps around the protein polypeptide backbone and coat it with negative charges to the protein in order to make sure the protein have the same negative charge/mass ion. The shape of the protein is now linearlized and denatured polypeptide How and where does SDS bind to protein Correct Answer-How? by hydrophobic interaction between dodceyl g...

Chapter 5: Proteins: Primary Structure Matching A) electrophoresis B) hydrophobic interaction C) enzyme-linked immunosorbent assay D) three-dimensional shape E) N-terminal amino acid F) negative charge G) nucleases H) chromophore I) foaming J) high level expression K) 2-mercaptoethanol L) positive charge M) cation exchange N) pI O) chymotrypsin P) C-terminal amino acid Q Sodium dodecyl sulfate 1. One of the reasons the primary structure is important for a protein is that it de...

Gel Electrophoresis Questions and Answers Already Passed What property of DNA allows it to migrate towards the positive electrode during gel electrophoresis? DNA is negatively charged due to its phosphate backbone, causing it to move towards the positive electrode when an electric field is applied. In gel electrophoresis, what role does the gel matrix play in the separation of molecules? The gel matrix acts as a sieve, allowing smaller molecules to pass through more easily while re...