Btec 6303 final exam - Study guides, Class notes & Summaries

What is the purpose of having rrnBT4 in an expression vector? - correct answerEnhances the stability of the GOI transcript Why must reverse transcriptase be used to create a eukaryotic expression library? - correct answerThe resulting cDNAs do not contain non-protein coding introns Which type of ligation-independent cloning technique takes advantage of the recombination between lambda phage attB and attP sequences in the vectors and in the DNA fragment to be inserted? - correct answerGatew...