Sodium dodecyl sulfate - Study guides, Class notes & Summaries

Ls 23L Master Final Stuff Verified Tests questions And Answers 2023

ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS ALL CORRECT Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exc...

Henderson-Hasselbach Equation pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography Separates sample based on size with smaller molecules eluting later. Ion-Exchange Chr...

ACS BIOCHEMISTRY EXAM QUESTIONS WITH COMPLETE SOLUTIONS Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution....

ASCP MB TEST VERIFIED SOLUTIONS 100% PASS What is biotin and digoxigenin most commonly used for in the lab? Why are they safer? Probe labeling (aka tagging nucleic acids so that they can be visualized). They are safer because they are nonradioactive. Name the three basic methods used to label a DNA probe: 1. End Labeling 2. Nick Translation 3. Random priming What is SDS? Name 3 of its applications in the lab. Sodium Dodecyl Sulfate. A detergent. 1. Denatures proteins. 2. Used i...

ACS BIOCHEMISTRY EXAM 200+ QUESTIONS WITH VERIFIED ANSWERS BY EXPERTS 2023 LATEST UPDATE. 1. Henderson-Hasselbach Equation: pH = pKa + log ([A-] / [HA]) 2. FMOC Chemical Synthesis: Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. 3. Salting Out (Purification): Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. 4. Size-Exclusion Ch...

LS23L Final Exam Review Questions And Answers Rated 100% Correct!!

ASCP MB TEST VERIFIED SOLUTIONS 100% PASS What is biotin and digoxigenin most commonly used for in the lab? Why are they safer? Probe labeling (aka tagging nucleic acids so that they can be visualized). They are safer because they are nonradioactive. Name the three basic methods used to label a DNA probe: 1. End Labeling 2. Nick Translation 3. Random priming What is SDS? Name 3 of its applications in the lab. Sodium Dodecyl Sulfate. A detergent. 1. Denatures proteins. 2. Used i...

Native-PAGE - Answer-A polyacrylamide gel electrophoresis method for proteins using NON-DENATURING conditions. Proteins keep their native charge and structure so they are separated based on charge and size. SDS-PAGE - Answer-A polyacrylamide gel electrophoresis method for proteins using DENATURING conditions. Sodium Dodecyl Sulfate denatures the proteins and gives the proteins a uniform charge. This allows them to be separated solely on mass, thus, you can estimate the protein's molecular ma...

pKa (chapter 3) correct answers measure of the tendency of a group to give up a proton (acidity); tendency decreases tenfold as pKa increases by one unit thioester bond (1) correct answers compounds with functional group C-S-CoA-C (eg, in acetyl-CoA) zwitterion (3) correct answers dipolar ion with both positive and negative groups but overall neutral charge; can act as either an acid or base Isoelectric point (pI) (chapter 3) correct answers the characteristic pH at which the net electr...