Restriction enzymes - Study guides, Class notes & Summaries

MCB 2050 Molecular Biology of the Cell - W21 Practice Midterm exam questions and answers tips with Dr. Yankulov University of Guelph PART A 1. Which of the following is NOT an essential component of a cloning vector? a. A unique restriction endonuclease cleavage b. A dominant selectable marker gene c. An origin of replication d. A Taq polymerase binding site 2. Methylation of DNA sequence is used how by bacteria? a. Methylation of DNA can inhibit cell division b. Methyla...

Biochemistry Quiz Latest Update Already Passed What is the primary role of phospholipids in cellular structures? Phospholipids form the bilayer of cell membranes, creating a barrier that separates the interior of the cell from the external environment. How do enzymes lower activation energy in biochemical reactions? Enzymes stabilize the transition state and provide an alternative reaction pathway, lowering the energy required for the reaction to proceed. What is a cofactor, an...

How can directional terms and regional terms help describe location in the body? - Directional Terms: locations of the body Regional Terms: Landmarks on the body What features of structure and function are common to all humans? (only do 4) - 4 tissue types, 23 pairs of chromosomes, 206 bones at maturity, DNA, Bipedal, (2 legs- not always true) highly evolved cerebral cortex, 24 ribs- 12 pairs, 4 chambered heart, lungs dependent on outside pressure, and little body hair. What are the main ...

Methylases - Answer adds methyl group to restriction sites on cell's own DNA (blocks cleavage) Silent mutation - Answer No change in amino acid sequence Nonsense mutation - Answer Introduction of stop codon Missense mutation - Answer Amino acid change at position Dispersive replication - Answer fragments of original DNA molecules are used as templates Reciprocal translocation - Answer exchange of one part of a chromosome for another (problems in meiosis) Re...

BIO 333 Exam 1 Questions And Answers 2024/2025 A+ Review what is a molecular standard - ANS-o A series of known DNA fragment sizes that are used to determine the sizes of unknown DNA fragments o aka DNA ladder why do we use molecular standards - ANS-o To determine unknown sizes of DNA how do we make a molecular standard? - ANS-o Digest lambda DNA using the HindIII restriction enzyme to generate the molecular weight standard what are restriction enzymes? - ANS-o Enzymes that cleave phosp...

Genetics final exam prep, (Answered) With Complete Verified Solutions 1. Place the steps of generating a gene - knockout mouse in order from the dis- ruption of the target gene to generat- ing homozygous knockouts. Suppose in 1. Disrupt target gene by insert- ing neo+ and link tk+ to disrupted gene 2. transfer disrupted gene into one experiment, the stem cells used are stem cells from a black mouse from a black coated mouse, and the earl3y. allow recombination with mouse embryo ...

Straighterline Micro 250 final prep | Questions and Correct Answers | Latest Update 2024/2025 | 100% PASS Which class of eukaryotic microorganisms can be subdivided into groups such as Mastigophora, Sarcodina, Ciliophora, and Apicomplexa? A. Helminths B. Algae C. Protozoa D. Fungi - Answer -Protozoa Genetic material can be excised from one chromosome and directly integrated into another viaA. conjugation. B. transformation. C. transduction. D. transposons. - Answer -transposons. W...

What modification neutralizes the charges on histones and looses up the interactions between histones and DNA? - Answer- ACETYLATION Two types of post-transcriptional modifications that take place in the mRNA of eukaryotes - Answer- the addition of a CAP at the 5' end of the transcript and the addition of a POLY A sequence at the 3' end of the message Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally - Answer- Hybridizes filter-bound DNA...

NR228 / NR-228 Exam 2 (Latest Update 2024 / 2025): Nutrition, Health & Wellness | Complete Guide with Questions and Verified Answers | 100% Correct - Chamberlain Question: What is the antidote for warfarin (Coumadin) Answer: vitamin k = dark leafy greens Question: What should not be taken with MAOIs? Answer: tyramines.. it causes severe hypertension = cured meats, paesturized foods, fermented foods, aged cheeses, pickled foods Question: What is good to take with iron?...

What are the steps of a southern blot? - ️️1. DNA is isolated and cut with restriction enzymes 2. Fragments are separated by gel electrophoresis 3. DNA fragments are exposed to a labeled probe (complementary DNA or RNA), complementary to region of interest What is RFLP? - ️️- Restriction Fragment Length Polymorphism - The number and location of restriction sites for a given restriction enzymes are not the same in all individuals What is the mapping difference between linear DNA f...