Dna electrophoresis - Study guides, Class notes & Summaries

Lab 3 Gel Electrophoresis Questions and Answers Graded A+ What would happen if you dropped your DNA sample outside the well? If you miss the well, your sample would float away in the buffer, and nothing will separate during the run! Why does DNA naturally "run away" from the negative end of the gel? DNA has a negative charge due to its phosphate backbone, so it’s repelled by the negative electrode and races toward the positive side. What do the DNA bands look like under U...

recombinant DNA correct answers DNA from 2 sources is known as procedure that leads to many copies correct answers gene cloning vector correct answers a carrier for DNA that is to be cloned plasmid, viral correct answers What are the 2 most common vectors? DNA ligase correct answers _______________________ must be used to permanently link the DNA before it is introduced into a host cell. restriction enzymes correct answers When performing gene cloning, the vector and the DNA of in...

MB ASCP Test Molecular Techniques (20-30%) Questions & Answers 2024 What are the 3 types of gel electrophoresis - ANSWER-Agarose Polyacrylamide Pulse Field What is gel electrophoresis - ANSWER-the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. which direction does the DNA flow in gel electrophoresis - ANSWER-due to DNA being negatively charged, it will migrate towards the positive end What determines how far...

MB ASCP Test Molecular Techniques (20-30%) Questions & Answers 2024 What are the 3 types of gel electrophoresis - ANSWER-Agarose Polyacrylamide Pulse Field What is gel electrophoresis - ANSWER-the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. which direction does the DNA flow in gel electrophoresis - ANSWER-due to DNA being negatively charged, it will migrate towards the positive end What determines how far...

MB ASCP Test Molecular Techniques (20-30%) Questions & Answers 2024 What are the 3 types of gel electrophoresis - ANSWER-Agarose Polyacrylamide Pulse Field What is gel electrophoresis - ANSWER-the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. which direction does the DNA flow in gel electrophoresis - ANSWER-due to DNA being negatively charged, it will migrate towards the positive end What determines how far...

Gel Electrophoresis Questions and Answers Graded A+ What is the relationship between gel concentration and the size of molecules being separated? Higher gel concentrations create smaller pores, which are more effective for separating smaller molecules, while lower concentrations allow for the separation of larger molecules. Describe how gel electrophoresis can be used to determine the purity of a DNA sample. By running a gel electrophoresis, one can observe the banding pattern of a D...

HBS End of Course Review Questions with Certified Solutions **How can directional terms and regional terms help specify body location?** Directional Terms: Indicate positions within the body Regional Terms: Refer to distinct landmarks on the body **What characteristics of structure and function are universal to all humans?** Four types of tissues, 23 pairs of chromosomes, 206 mature bones, DNA, Evolutionarily advanced cerebral cortex, 24 ribs (12 pairs), Four-chambered heart, Lu...

the application of science to clinical medicine is known as a. Biotechnology b. Biomedical Science c. Microbiology d. Anatomy and Physiology correct answers b. Biomedical Science Which of the following is NOT a step in processing a crime scene? a. Sketch the scene b. Photograph the scene c. Interview witnesses d. Call the coroner correct answers d. call the coroner Why is it important that investigators wear the correct PPE? a. To protect the crime scene from contaminatio...

PLTW HBS Final Exam Review Questions with Verified Answers **Location on the body where blood is typically drawn for routine purposes?** Arm veins **A structure resembling a basin in the skeleton of many vertebrates, formed by the pelvic girdle along with the sacrum and often coccygeal vertebrae, consisting of the hip bones on each side and front, and completed by the sacrum and coccyx behind?** Pelvis **The longest bone in the upper arm or forelimb, extending from the shoulder t...

Electrophoresis Steps Latest Update Graded A+ What is the first step in preparing for gel electrophoresis? The first step is to prepare the agarose gel by dissolving agarose powder in a buffer solution and heating it until it melts. How do you make sure the gel has wells for loading samples? You insert a comb into the gel mold while the agarose is still liquid; once it cools, the comb creates wells for the samples. Why is it important to let the gel solidify before running the ex...