Acs biochemistry exam 1 - Study guides, Class notes & Summaries

BIOCHEMISTRY ACS EXAM QUESTIONS WITH 100% CORRECT ANSWERS Unfolds proteins and gives them uniform negative charge. - answerSDS Reacts with the N-terminus of the protein to produce a 2,4-dinitrophenol derivative that labels the first residue. Can repeat hydrolysis to determine sequential amino acids - answerFDNB Reduces disulfide bonds. - answerDTT Adds carboxymethyl group on free -SH groups. Blocks disulfide bonding. - answerIodoacetate Bonds between two -SH groups that form between 2°...

ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS Henderson-Hasselbach Equation - answerpH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - answerUsed in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - answerChanges soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - answerSeparates sample b...

ACS BIOCHEMISTRY EXAM 200+ QUESTIONS WITH VERIFIED ANSWERS BY EXPERTS 2023 LATEST UPDATE. 1. Henderson-Hasselbach Equation: pH = pKa + log ([A-] / [HA]) 2. FMOC Chemical Synthesis: Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. 3. Salting Out (Purification): Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. 4. Size-Exclusion Ch...

ACS Biochemistry Practice Final, FGCU, Beharry, Coticone Exam with 100% Correct Answers 1. A weak acid, HA, has a pKa of 4.11. a) At pH 7.11, what will the ratio of conjugate base to acid be? (Henderson-Hasselbach equation: pH=pKa + log [A-]/[HA]) b) Based on answer to "a," is there more HA or A- at that pH? c) At pH 2.11, what will the ratio of conjugate base to acid be? d) Based on answer to "c," is there more HA or A- at that pH? e) At pH 4.11, what will the ratio of conjugate ba...

ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS ALL CORRECT Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exc...

ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS 2024 what are nucleotides? nucleotides are the monomer unit for nucleic acids and are composed of nitrogenous bases, 5 carbon sugars and a phosphate group. nucleic acids are bipolymers of _____ nucleotide monomers what is DNA and what is its stability? DNA is more stable than RNA and carries genetic information, and is double stranded. what is RNA and what is its stability? RNA is less stable and is an intermediate in expression of genetic in...

Biochemistry ACS Exam Questions with All Correct Answers What are nucleotides? - ANSWER the monomer unit for nucleic acids and are composed of nitrogenous base, 5-carbon sugar & phosphate group Nucleic Acids are biopolymers categorized as: - ANSWER DNA RNA DNA - ANSWER carrier of genetic information RNA - ANSWER intermediate in gene expression (not all) can form secondary structure - tRNA 4 types of RNA - ANSWER tRNA rRNA siRNA mi...

ACS BIOCHEMISTRY EXAM | Questions & Answers (Graded A+) |100% VERIFIED Latest Update 2023 Henderson-Hasselbach Equation FMOC Chemical Synthesis Salting Out (Purification) Size-Exclusion Chromatography Ion-Exchange Chromatography Hydrophobic/Reverse Phase Chromatography Affinity Chromatography SDS-PAGE SDS Isoelectric Focusing FDNB (1-fluoro-2,3-dinitrobenzene) DTT (dithiothreitol) Iodoacetate Homologs Orthologs Paralogs Ramachandran Plot Glycine Ramachandran Plot Proline Ramac...

ACS Biochemistry 2023-2024 Exam Questions with All Correct Answers Metabolism (catabolism and anabolism) - ANSWER Metabolism: sum of total chemical reactions in an organism, also the method by which cells extract and use energy from their environment. Catabolism: The process by which stored nutrients and ingested foods are converted to a usable form of energy. It produces simple products CO2, H2O, NH3, and building blocks such as sugars and fats that are used in anabolism. Anaboli...

Biochemistry ACS Final Exam Questions and Answers What is the difference between an apo and holoprotein - ANSWER Apo = protein part of an enzyme without its characteristic prosthetic group (cofactor is not bound) Holo = apoprotein with its prosthetic group (cofactor bound) Many amino acids exist as what isomer? - ANSWER L Most alpha amino acids are _____, expect for glycine. All amino acids have ______ unique to itself. - ANSWER Chiral, R-group/4th substituent W...