Cmmb 403 final exam - Study guides, Class notes & Summaries

CMMB 403 Final Exam Actual Questions and Answers with complete solution what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - answer- nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR - answerendonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - answer1. non-homologous...

CMMB 403 final examwhat does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - correct answer - nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR - correct answer endonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - correct answer 1. non-homologous end joining (NHEJ) - n...

CMMB 403 Final Exam Actual Questions and Answers with complete solution 72 hours (8 cells) (BEFORE BLASTOCYST STAGE) - answerwhat is the human MZT Mid-blastula transition/maternal-zygotic transition - answerwhen the transcription of zygotic genes begins and maternal mRNAs are degraded, the cell cycle lengthens, gap stages are added (before is only M and S) and the synchronicity of cell division is lost. - embryo runs on maternal resources (mRNAs and proteins in the egg @ time of fertilizat...

CMMB 403 Final Exam Actual Questions and Answers with complete solution Which animal's development is chick development most similar to compared to other species? - answerMost similar to humans than other species talked about in lecture (like amphibians and flies) What is the Nieuwkoop Centre (NC)? When does it emerge during embryo development? - answerCentre that induces the formation of the organizer and later the dorsal mesoderm; emerges during the late blastula stage How does the fi...

what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - - nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR - endonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - 1. non-homologous end joining (NHEJ) - naturally try to repair - imperfect, creates indels causing frameshi...

CMMB 403 Final Exam Study guide 2with complete solution

CMMB 403 Final Exam with complete solution Mid-blastula transition (MBT) - answertransition from synchronous to asynchronous cell division Blastocoel - answercavity during development around time of MBT, fluid-filled space; future anus (frog) and future mouth (sea urchin) Gastrulation - answerrearrangement of cell layers during early embryo development; endoderm, mesoderm and ectoderm rearrangment Fate map - answerused at specific stage of embryo development that can tell what cells will...

CMMB 403 Final Exam Questions With Correct Answers What are the origins of the optic vesicle and the lens? - answeroptic vesicle = forebrain (neural ectoderm) lens = surface epidermis --> placode Describe the process of lens induction - answer1. the optic vesicle (forebrain) induces thickening of the surface epidermis (prospective lens placode) 2. the optic vesicle pinches in to form the optic cup and the lens invaginate as well (lens placode comes into contact w/ the optic vesicle) ...

CMMB 403 Final Exam with complete solution Mid-blastula transition (MBT) - answertransition from synchronous to asynchronous cell division Blastocoel - answercavity during development around time of MBT, fluid-filled space; future anus (frog) and future mouth (sea urchin) Gastrulation - answerrearrangement of cell layers during early embryo development; endoderm, mesoderm and ectoderm rearrangment Fate map - answerused at specific stage of embryo development that can tell what cells will...

CMMB 403 final Exam New Year 2024| Questions and Answers| Certified Quality Graded A+ what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR endonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 1. non-homologous end jo...