Bcmb 406b - Study guides, Class notes & Summaries

what variables are considered in primer design length, sequence, %GC, Tm, Ta, 3'end stability, 5' end stability, secondary structures why is the formation of secondary structures in primers especially undesirable reduces primer available to use to make product Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More standard denaturation temp and time (initial and denaturation) 94C 1-5min initial 20-45 sec...

BCMB 406B - Lab 2 Test Study Guide ...

0.1 ng vs. 1 ng pET28a w/ Kan selection - Answer - Showed that the amount of pET28a plasmid DNA doesn't affect the transformation efficiency 0.1 ng vs 0.1 ng (1/10 dilution) pUC19 w/ Kan selection - Answer - Showed that the amount of pUC19 present influenced the transformation efficiency Untreated CBM26-3 PCR product - Answer - Shows that the WT DNA can be transformed and grow on a plate without the DpnI treatment

BCMB 406B Test 1Latest Update ...

BCMB 406B - Lab 1 Exam Questions With Correct Answers The success of a PCR reaction is mostly dependent on what? - answerwell designed primers Primer design can also be applied to what? - answer- probes in Southern and Northern blots - DNA sequencing - microarray design What else can affect the success of a PCR reaction? - answer- Quality of the template DNA - cycling conditions - Specificity and stability of the PCR primers What are key criteria in primer design? - answer- Primer length...

Reasons for sequence errors within PCR products - Answer Low fidelity of DNA polymerase, excess Mg2+ concentration, unbalanced dNTP concentrations, high number of cycles, UV-damaged DNA, or sequencing error Reasons for sequence error at termini of PCR products - Answer Problematic primer design, low primer quality, contaminating nucleases, UV-damaged DNA, or sequencing error