What is crispr do - Study guides, Class notes & Summaries

CRISPR-Cas9 Complete Study Guide Exam 2024. What is CRISPR-Cas9 used for? - CORRECT ANSWER Recently developed method for making precise and targeted changes in the genome of a living cell What are the advantages of using CRISPR-Cas9? - CORRECT ANSWER Fast, efficient, works in many different species, not limited by bg strain, doesn't produce chimeras, no molecular scar left behind Because CRISPR-Cas9 is so use friendly what has it done for research? - CORRECT ANSWER Open all kinds of ...

Molecular Diagnostics Chapter 5 Exam What type of restriction enzyme is commonly used in the laboratory? - ANSWERtype II, 4-6 bp recognition sites restriction map - ANSWERdiagram that shows the lengths of fragments between restriction sites in the strand of DNA If you cut a linear fragment of DNA with a restriction enzyme at three sites, how many fragments do you have? What about if you cut a plasmid at three sites - ANSWER4 fragments, 3 fragments How do you perform restriction mapping? ...

Dimitri Ivanovsky Russian Botanist -- discovered disease was infectious (sap) Martinus Beijerinck Coined term "virus" to indicate non-bacterial Example of Viral Diseases Small Pox (Edward Jenner -- derived vaccine from cowpox) Poliovirus (causes poliomyelitis, paralysis, still exists all over the world mostly poorer nations ) What is hindering eradication of Polio -Cost of vaccine manufacturing -Inefficient supply -Limited vaccine available for supplementary immun...

1. The unpaired nucleotides produced by the action of restriction enzyme are referred to as - Anssticky ends 2. What information cannot be obtained from the sequence of a gene? - Answhether the gene is methylated 3. What is the polymerase chain reaction (PCR)? - Ansa method to amplify a fragment of DNA 4. Comparison of the sequences of the same gene across species can give some insight into the existence of a common ancestor with that gene. - Anstrue 5. The Taq enzyme is a type of DNA p...

Genetics Second Exam Practice Test 150 Questions with Verified Answers Which organism has polycistronic mRNA? (eukaryotic or prokaryotic) - CORRECT ANSWER Prokaryotic Do prokaryotes undergo splicing/processing? - CORRECT ANSWER No Where are attenuater sequences found? (what type of organism and what class of operons?) - CORRECT ANSWER In prokaryotic amino acid operons Do prokaryotic mRNA ALWAYs encode trans-acting factors? - CORRECT ANSWER No Which of the following statements is t...

What is the method of SCNT? correct answers SCNT is Somatic cell nuclear transfer Why were M9 and MOPS mediums used for E.Coli growth? correct answers They both have contain glucose which led to increased levels of alpha-ketoglutarate Currently, ________ therapy is the only method of management of mitochondrial diseases. correct answers Supportive Congenital Insensitivity to Pain (CIP) is a rare disease caused by a genetic mutation that affects an individual's nociceptive neurons, whic...

CRISPR Cas-9 Semester 1 Questions With Correct Actual Answers 2024. How many nucleotides long is the guiding region of the sgRNA? - CORRECT ANSWER 20 nucleotides Does the sgRNA bind to the PAM? - CORRECT ANSWER Yes, there is a spacer on the sgRNA that is within the Cas9 protein that binds to the protospacer sequence, opening up the double helix Where does Cas9 cut the target DNA relative to the protospacer sequence? - CORRECT ANSWER It cuts the DNA 3 nucleotides upstream of the PAM De...

CRISPR Cas Written Exam Questions With 100% Correct Answers 2024. How many nucleotides long is the guiding region of the sgRNA? - CORRECT ANSWER 20 nucleotides Does the sgRNA bind to the PAM? - CORRECT ANSWER Yes, there is a spacer on the sgRNA that is within the Cas9 protein that binds to the protospacer sequence, opening up the double helix Where does Cas9 cut the target DNA relative to the protospacer sequence? - CORRECT ANSWER It cuts the DNA 3 nucleotides upstream of the PAM Descr...

CRISPR-Cas9 Final Practice Exam Questions 2024.What is CRISPR-Cas9 used for? - CORRECT ANSWER Recently developed method for making precise and targeted changes in the genome of a living cell What are the advantages of using CRISPR-Cas9? - CORRECT ANSWER Fast, efficient, works in many different species, not limited by bg strain, doesn't produce chimeras, no molecular scar left behind Because CRISPR-Cas9 is so use friendly what has it done for research? - CORRECT ANSWER Open all kinds of re...

CRISPR-Cas9 Complete Study Guide Practice Exam 2024. What is CRISPR-Cas9 used for? - CORRECT ANSWER Recently developed method for making precise and targeted changes in the genome of a living cell What are the advantages of using CRISPR-Cas9? - CORRECT ANSWER Fast, efficient, works in many different species, not limited by bg strain, doesn't produce chimeras, no molecular scar left behind Because CRISPR-Cas9 is so use friendly what has it done for research? - CORRECT ANSWER Open all kin...