Cloning vectors - Study guides, Class notes & Summaries

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

immunological assay method correct answers What method do we use when we want to find a protein we are interested in but we don't know the exact genetic sequence? antibody binding correct answers What mechanism does the immunological assay method use to highlight the desired protein? primary antibody correct answers In the immunological assay method, the ____ binds to the target protein. secondary antibody correct answers In the immunological assay method, the ____ reacts with the ...

BS 161 FINAL EXAM WITH COMPLETE SOLUTIONS ( 3 VERSIONS) VERSION 1 Barbara McClintock, who achieved fame for discovering that genes could move within genomes, had her meticulous work ignored for nearly four decades, but eventually won the Nobel Prize. Why was her work so distrusted? A)She worked only with maize, which was considered "merely" a plant. B)Geneticists did not want to lose their cherished notions of DNA stability. C)She allowed no one else to duplicate her work. ...

GCD Quiz 7/8 Practice 69 Questions with Verified Answers Which of the following may be used as a vector in a gene-cloning experiment? a.mRNA b.Plasmid c.Virus d.Both b and c - CORRECT ANSWER d The restriction enzymes used in gene-cloning experiments _______, which generates sticky ends that can _______. the DNA, enter bacterial cells the DNA, hydrogen bond with complementary sticky ends late DNA, enter bacterial cells late DNA, hydrogen bond with complementary sticky ends - CORRE...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

The _______ number system allows digital devices to represent virtually any number simply by using 0s and 1s. (Unit 1) Binary Most graphics software uses a process called pixel _________ to create new pixels by averaging the colors of nearby pixels. (Unit 1) Interpolation Brainpower Read More Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:00 Full screen MIDI files are less compact than digital audio files. (Unit 1) False Which o...

The _______ number system allows digital devices to represent virtually any number simply by using 0s and 1s. (Unit 1) Binary Most graphics software uses a process called pixel _________ to create new pixels by averaging the colors of nearby pixels. (Unit 1) Interpolation Brainpower Read More Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:00 Full screen MIDI files are less compact than digital audio files. (Unit 1) False Which o...

Biochem Engineering: Recombinant DNA Tech Exam Questions With Verified Answers. Ways to cut DNA - answer1. chemical cleavage (random) 2. shearing (random) 3. restriction enzymes restriction endonucleases - answercut both strands of DNA duplex at a specific place restriction mapping - answerfor determining the locations of restriction endonuclease sites within a segment of DNA sum of fragment sizes equals... - answersize of unfragmented DNA molecule partial digestion - answerDNA incubat...

EXAM A-49 SUPERVION OF STORAGE HANDLING AND USE OF AEROSOL ACTUAL QUESTIONS AND ANSWERS WRITTEN AT CHAMBRLIAN COLLEGE OF NURSING AND WAS UPDATED ON JUN 27 2024 What feature is commonly seen in the sequences recognized by type II restriction enzymes? - ANS--Solution: The recognition sequences are palindromic, and 4−8 base pairs long. What normal role do restriction enzymes play in bacteria? How do bacteria protect their own DNA from the action of restriction enzymes? - ANS--Solution: ...

BS 161 FINAL EXAM WITH COMPLETE SOLUTIONS ( 3 VERSIONS) VERSION 1 Barbara McClintock, who achieved fame for discovering that genes could move within genomes, had her meticulous work ignored for nearly four decades, but eventually won the Nobel Prize. Why was her work so distrusted? A)She worked only with maize, which was considered "merely" a plant. B)Geneticists did not want to lose their cherished notions of DNA stability. C)She allowed no one else to duplicate her work. ...