Bio 219 final exam - Study guides, Class notes & Summaries
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Bio 219 Final Exam Review Questions with Correct Answers
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Bio 219 Final Exam Review Questions with Correct Answers
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Bio 219 Final Exam 100% Verified!
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Purpose of this experiment - ANSWERto create a genomic library of A. fischeri 
to create a restriction map of the plasmid - containing lux operon 
 
Overview of experiment in order - ANSWER1. Isolate chDNA from A. fischeri 
2. Analyze purity and determine concentration 
3. Restriction digestion / Shotgun Cloning 
4. Ligation of insert and vector 
5. Transformation / Screening 
6. Re-isolation (Plasmid mini-prep) 
7. Restriction mapping 
 
Characteristics of A. fischeri - ANSWER- gram negative ...
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Bio 219 Final Exam 100% Verified!!
- Exam (elaborations) • 8 pages • 2024
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Purpose of this experiment - ANSWERto create a genomic library of A. fischeri 
to create a restriction map of the plasmid - containing lux operon 
 
Overview of experiment in order - ANSWER1. Isolate chDNA from A. fischeri 
2. Analyze purity and determine concentration 
3. Restriction digestion / Shotgun Cloning 
4. Ligation of insert and vector 
5. Transformation / Screening 
6. Re-isolation (Plasmid mini-prep) 
7. Restriction mapping 
 
Characteristics of A. fischeri - ANSWER- gram negative ...
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BIO N110 LEON FINAL EXAM (SPRING 22) Sec A (219 questions and answers).
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BIO N110 LEON FINAL EXAM (SPRING 22) Sec A (219 questions and answers).
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Bio_2000_01.pdf
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Bio-2000 
An Introduction to Biology 
Contacts 
• Dr. Vladimir Poltoratsky 
• Department of Biology 
• Room SAH 219 
• Email: 
• Office hours: Tu, Fr 9:30 – 11:00 AM, 
• or by appointment 
Course Policies 
1. All material covered in class is considered 
exam material 
2. Be courteous to your colleagues: don’t be 
late, and do not carry on private 
conversations in class 
3. No cell phones usage in the classroom. If you 
feel you must use your phone during class 
please leave th...
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