Bcmb 406b - Study guides, Class notes & Summaries

Purpose of SDS-NaOH and Sodium Acetate in Alkaline lysis method? - Answer SDS- NaOH: achieves cell lysis - Chromosomal DNA remains in a high molecular weight form but is fully denatured Sodium Acetate Solution: neutralizes solution - Chromosomal DNA randomly base pairs and becomes insoluble - High salt concentration precipitates SDS-protein complexes and high molecular weight DNA

BCMB 406B Midterm 1 Test Latest Update ...

what two criteria need to be meet when picking a replacement residue in SDM - Answer remove charge of functional group of original residue be similar or smaller in size What are the most significant problems in primer design - Answer secondary structures (loses primer), 3' end stability (mispriming), Tm difference (hard to set up) Primer length - Answer 18-22bp

BCMB 406B Midterm 1 Lab 1 Test Latest Update ...

BCMB 406B Midterm 1 lab 1 Exam Questions with Detailed, (COMPLETE ANSWERS), 2024; 100% TRUSTED Complete, trusted solutions and explanations

what variables are considered in primer design - length, sequence, %GC, Tm, Ta, 3'end stability, 5' end stability, secondary structures why is the formation of secondary structures in primers especially undesirable - reduces primer available to use to make product standard denaturation temp and time (initial and denaturation) - 94C 1-5min initial 20-45 sec std annealing temp and time - temp varies between protocols, 30s std elongation temp and time - temp depends on polymerase u...

The success of a PCR reaction is mostly dependent on what? well designed primers Primer design can also be applied to what? - probes in Southern and Northern blots - DNA sequencing - microarray design Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More What else can affect the success of a PCR reaction? - Quality of the template DNA - cycling conditions - Specificity and stability of the PCR primers ...

Glycoside hydrolases Hydrolyze the glycosidic bond between 2 or more carbohydrates or carbohydrate-protein bonds Carbohydrate Binding Modules - Amino acid sequence within a carbohydrate-active enzyme with a discreet fold that has carbohydrate binding activity - Maintains the interaction of the enzyme with its substrate Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More Vector NTI Advance - Allows you to...

what variables are considered in primer design length, sequence, %GC, Tm, Ta, 3'end stability, 5' end stability, secondary structures why is the formation of secondary structures in primers especially undesirable reduces primer available to use to make product Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More standard denaturation temp and time (initial and denaturation) 94C 1-5min initial 20-45 sec...