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WGU Microbiology D311 - OA Prep 2024 (Staining And Immunology): Q’s And A’s
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WGU Microbiology D311 - OA Prep 2024 (Staining And Immunology): Q’s And A’s
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WGU Microbiology D311 - OA Prep 2024 (Staining AndImmunology): Q’s And A’s
What is a Basic Stain? Right Ans - If the chromophore is the positively
charged ion
Waht is an Acidic Stain? Right Ans - if the negative ion is the chromophore
What is a negative stain? Right Ans - A stain that is absorbed by the
background but not by the cells or organisms in the specimen.
What does a negative stain produce? Right Ans - An outline or silhouette of
the organisms against a colorful background.
What is Gram Stain used for? Right Ans - It is a differential staining
procedure that involves multiple steps to distinguish between bacteria with
different types of cell walls.
How many steps are involved in Gram Stain? Right Ans - Gram Stain
involves multiple steps.
What is Acid-Fast Staining? Right Ans - Acid-fast staining is a differential
staining technique and is able to differentiate two types of gram-positive cells:
those that have waxy mycolic acids in their cell walls, and those that do not.
uses carbolfuchsin as the primary stain.
What happens with Acid-Fast Staining? Right Ans - The waxy, acid-fast cells
retain the carbolfuchsin even after a decolorizing agent (an acid-alcohol
solution) is applied. A secondary counterstain, methylene blue, is then
applied, which renders non-acid-fast cells blue.
What is the purpose of using heat in the endospore stain technique? Right
Ans - To push the primary stain (malachite green) into the endospore
What is the primary stain used in endospore staining? Right Ans -
Malachite green
, How are endospores differentiated from the rest of the cell in endospore
staining? Right Ans - Endospores retain the green stain while the rest of the
cell is counterstained pink with safranin
What is a capsule stain? Right Ans - A negative staining technique where
the dye stains the background but not the capsules, creating halos around the
cell borders.
How does capsule staining work? Right Ans - The dye in capsule staining
does not penetrate the capsules, resulting in halos around the cell borders
without the need for heat-fixing the specimen.
Primary Stain Right Ans - in differential staining techniques, refers to the
first dye added to the specimen
Mordant Right Ans - a chemical added to a specimen that sets a stain
Decolorizing Agent Right Ans - a substance that removes a stain, usually
from some parts of the specimen
What is a counterstain? Right Ans - A secondary stain that adds contrasting
color to cells
What does a decolorizing agent do in staining? Right Ans - Washes out the
primary stain from cells
Wet Mount Right Ans - a slide preparation technique in which a specimen
is placed on the slide in a drop of liquid
Fixation Right Ans - the process by which cells are killed and attached to a
slide
Smear Right Ans - a thin layer of a specimen on a slide
Staining Right Ans - the addition of stains or dyes to a microscopic
specimen for the purpose of enhancing contrast
Positive Stain Right Ans - a stain that colors the structure of interest
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