MBB 331 Midterm 2 UPDATED ACTUAL Questions and CORRECT Answers
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Course
MBB 331
Institution
MBB 331
MBB 331 Midterm 2 UPDATED ACTUAL
Questions and CORRECT Answers
What is Homologous recombination - CORRECT ANSWER- Physical exchange,
crossover, of homologous DNA.
What are the Similarities between strand invasion, Holliday Junction, and resolution. -
CORRECT ANSWER- all require a dsDNA break....
MBB 331 Midterm 2 UPDATED ACTUAL
Questions and CORRECT Answers
What is Homologous recombination - CORRECT ANSWER✔✔- Physical exchange,
crossover, of homologous DNA.
What are the Similarities between strand invasion, Holliday Junction, and resolution. -
CORRECT ANSWER✔✔- all require a dsDNA break.
Describe Recombination event - CORRECT ANSWER✔✔- 1)Double strand break DNA
2) Homologous alignment w 2nd DNA duplex
3)Generate 3' overhang
4)Strand invasion and repair of 3'
5)Branch migration and formation of recombination intermeidiate with Holliday Junction.
6)Resolution(cut) vertical or horizontal
What is the Chi site sequence - CORRECT ANSWER✔✔- -A short sequence of DNA in the
bacterial genome that increases the likely hood of recombination event occurring
-Recognized by RecC
Describe the function of RecA - CORRECT ANSWER✔✔- -promotes strand invasion and
displacement of complementary strand to generate D loop
-Coats ssDNA
-Recruited by RecB (3'-5' helicase)
-scans for homology with paired duplex
-5'-3' assembly
Describe the Branch Migration Process - CORRECT ANSWER✔✔- -RuvA and RuvB
-RuvA binds the junction
-RuvB pump DNA through the junction.
-RuvC Cleaves specific DNA strands at Holliday Junction and finish recombination.
, What proteins are involved in DSB in Eukaryotes? - CORRECT ANSWER✔✔- -Mediated
by Spo11 and MRX complex( homologs to RecBCD, RecA, RuvA,B,C)
What does RecBCD complex do? - CORRECT ANSWER✔✔- -initiates recombination
-processes broken DNA
-RecD 5'-3' helicase
-RecB 3'-5' helicase ( degrades both strands)
-RecC recognizes Chi site
-
Define site-specific recombination - CORRECT ANSWER✔✔- Recombination between
DNA molecules that do not share long regions of homology but do contain short regions of
homology specifically recognized by the recombination enzyme.
Define Transposition - CORRECT ANSWER✔✔- -Mobile elements that can move from one
location to another on a chromosome.
-No selectivity w respect to DNA sequence.
Define Recombination Site - CORRECT ANSWER✔✔- -2 flanking inverted repeats
- 1 core sequence
- Recomb only happens when 2 cores are identical
- if opposite orientation - Inversion
- if same orientation - Deletion and insertion
Define composite transposons (Tn) - CORRECT ANSWER✔✔- Have extra genes (
antibiotic resistance). Surrounded by insertion sequences.
What are the Types of transposons - CORRECT ANSWER✔✔- -LTR retrotransposon:
directed repeats (replicate via RNA intermediate)
-Non - LTR retrotransposon, no repeats(replicate via target primed mechanism)
-Inverted repeats
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