Molecular Cell Biology Chapter 6 Exam Questions and Correct Answers (Graded A+)
Organisms that are considered polyploid - Answers more than two copies of each chromosome
Which of the following tests is used to determine if different recessive mutations are in the same gene? - Answers complementat...
Organisms that are considered polyploid - Answers more than two copies of each chromosome
Which of the following tests is used to determine if different recessive mutations are in the same gene? -
Answers complementation test
The chemical mutagen ethylmethane sulfonate (EMS) was used to treat cells, and after 24 hours of
incubation, DNA was isolated from these cells and sent for sequencing. Based on the DNA sequence
from untreated cells, those treated with EMS showed several of which of the following conversions? -
Answers G-C to A-T
Crossing of a homozygous wild type with a mutant that is heterozygous for a dominant mutation will
result in F1 progeny of which - Answers half show the wild-type phenotype and half show the mutant
phenotype
A mutation in one gene that counteracts the effects of a mutation in another gene is known as a -
Answers suppressor mutation
A polylinker increases the versatility of a DNA plasmid because It contains - Answers a DNA recognition
sequences for several different restriction enzymes
Construction of a cDNA library involves several steps, the first usually involves - Answers isolating total
RNA from the organism or cell type of interest
Sequencing methods were used to test the integrity of the cDNA library and when this was done you
found many of the sequences were those found in introns. After troubleshooting you determined that
the reason these intronic sequences were present was because during the construction of this cDNA
library: - Answers genomic DNA was present
Which of the following series of steps does the polymerase chain reaction follow in order to amplify
DNA in a test tube? - Answers DNA denaturation, primer annealing, primer elongation
To see if your polymerase chain reaction was successful and it amplified the right sequence of interest,
you electrophorese the products from the reaction on an agarose gel. after staining the gel you find that
there are two bands of different sizes. Which one of the following is a possible explanation for these
results? - Answers The primers annealed to two different templates
Which of the following enzymes will produce a blunt end (the cut site us indicated by the * in the
recognition sequence)? - Answers EcoRV(GAT*ATC)
DNA ligase - Answers b and c
b) forms a phosphodiester bond
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