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BIO 209 questions and answers.
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BIOLOGY 209 Mini-Exams Questionsand Answers.
Match the microbe with the BSL precautions that should be used to work
with it.
1. Yeast
2. Staphylococcus aureus that causes MRSA
3. The virus that causes HIV
4. The Ebola virus - Correct Answers 1. BSL-1
2. BSL-2
3. BSL-3
4. BSL-4
Check all the personal safety equipment required for a BSL-2 laboratory. -
Correct Answers -safety glasses when handling liquid cultures
-lab coats
-closed-toed shoes
-gloves when using hazardous chemicals or staining
Which of the following are true of Biosafety Level - 2 (BSL-2)? - Correct
Answers -If you work in a lab that is designated a BSL-2, the microbes
there pose moderate hazards to laboratorians and the environment. The
microbes are typically indigenous and associated with diseases of varying
severity.
-Appropriate personal protective equipment (PPE) is worn, including lab
coats and gloves. Eye protection and face shields can also be worn, as
needed.
-A microbe that is typically worked with at a BSL-2 laboratory is
Staphylococcus aureus.
-BSL-2 requirements are more stringent than BSL-1 requirements.
Which microbe is a risk level-4 and requires biosafety level 4 (BSL-4) to
work with it? - Correct Answers Ebola virus
Which safety precaution are NOT needed for a BSL-1 laboratory? -
Correct Answers Locking doors, Bio Safety Cabinet
,Which of these are required to participate in person in microbiology
laboratory? - Correct Answers all the above: closed toe shoes, lab coat,
disposable gloves
Which of the following is NOT true? - Correct Answers It is not necessary
to heat loops before using them for streak plate experiments.
Chose all that apply: What method(s) does an autoclave use to sterilize lab
equipment? - Correct Answers Heat, Pressure
When subculturing from a broth culture to a plate, at what points should
you flame the loop? - Correct Answers Before obtaining a loopful of
inoculum from the broth and after streaking the inoculum onto the plate
When inoculating a broth from a colony, how long should you hold the loop
in the heat of the microincinerator (or Bunsen burner)? - Correct Answers
until the loop turns red
How does the transfer from an E. coli broth culture to broth media differ
from the transfer from an E. coli broth culture to slant media? - Correct
Answers The loopful of culture is plunged into the broth tube but spread
over the surface of the slant tube.
You are transferring a culture to a new tube using aseptic technique.
Arrange the following steps in the proper order.
-Loosen the caps of both culture tubes
-sterilize the loop in the micro incinerator, and let it cool down
-Remove the cap from tube 1 and retrieve a small amount of bacteria
-Place the cap on tube 1
-Remove the cap from tube 2 and inoculate the media
-Remove the loop from tube 2 and replace the cap
-Re-sterilize the loop in the micro incinerator - Correct Answers 1, 2, 3, 4,
5, 6, 7
The autoclave kills bacteria using - Correct Answers Heat and Pressure
Select the scenario when a quadrant streak plate would be useful. -
Correct Answers Determining the number of different microbes growing in
a patient's wound from a swab sample of the wound AND Identifying
contamination in a culture.
, What steps would you take in order to grow a pure culture of the red
organism? - Correct Answers Take a red colony and perform a second
streak plate (QSP).
Moving what part of a microscope will bring the specimen into focus? -
Correct Answers up/down movement of mechanical stage
What has the same refractive index as glass? - Correct Answers
immersion oil
The nosepiece of the microscope holds the ____________. - Correct
Answers objective lenses
What is the function of immersion oil? - Correct Answers Decreases light
refraction and improve resolving power
How many lenses are used at the same time when you view a specimen
using a compound light microscope? - Correct Answers two
Moving what part of a microscope will bring the specimen into focus? -
Correct Answers up/down movement of mechanical stage
When you view a specimen using the 40x objective, what is your total
magnification? - Correct Answers 400x
You want to move your microscope slide to the focal point of the objective
lense, so that you have the clearest image. Which direction should you
adjust the stage? - Correct Answers Up and Down
You perform a stain by applying an acidic dye to a bacterial smear. Predict
what you will see when you visualize the stained bacteria under the
microscope. - Correct Answers The bacteria will be visible as a white area
against a stained background.
Most bacteria are pigmented and easy to see in their natural state under
the microscope. - Correct Answers False
What characteristic should a perfect bacterial smear have? - Correct
Answers Thin whitish layer that you can read newsprint through
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