gel electrophoresis Questions And Answers Already Graded A+
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Course
Gel electrophoresis
Institution
Gel Electrophoresis
What purpose does the buffer serve in gel electrophoresis? - ️️It maintains pH (at 7
which is neutral) and conducts electricity which allows the DNA fragments to migrate
through the agarose gel
What does "phoresis" mean? - ️️To transmit (move location, migrate)
What three gel ingredient...
gel electrophoresis
What purpose does the buffer serve in gel electrophoresis? - ✔️✔️It maintains pH (at 7
which is neutral) and conducts electricity which allows the DNA fragments to migrate
through the agarose gel
What does "phoresis" mean? - ✔️✔️To transmit (move location, migrate)
What three gel ingredients are required for gel electrophoresis? - ✔️✔️Agarose, buffer,
and distilled H2O
What is agarose? - ✔️✔️A sugar found in seaweed
What is a characteristic of agarose? - ✔️✔️It is gelatin-like
What purpose does agarose serve in gel electrophoresis? - ✔️✔️It is the matrix
(obstacle course) for DNA fragments (it separates them by size)
What is the buffer? - ✔️✔️A salt solution
What is the purpose of gel electrophoresis? - ✔️✔️To separate DNA fragments based
on their size using electricity
What purpose does the distilled H2O serve in gel electrophoresis? - ✔️✔️It is the
solvent
Why does the H2O need to be distilled? - ✔️✔️It contains no chlorine or other
contaminants that could damage DNA
How is the agarose power measured? - ✔️✔️On an electronic balance, after zeroing
out the weighing tray
How is the distilled H2O measured? - ✔️✔️In a graduated cylinder (measured to the
meniscus)
What equipment is required for gel electrophoresis? - ✔️✔️Casting tray (baby gates,
combs), chambers, lids, power sources
What is the process of gel electrophoresis? - ✔️✔️The DNA is loaded into wells formed
by combs in the agarose gel, buffer is added, then an electric current is applied and the
DNA migrates to the bottom
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