Briefly summarize how gel electrophoresis is used to separate molecules - ️️when
the molecules are placed in the individual wells they are all at the same distance
because all the wells were created with the same comb. Gel electrophoresis is applying
an electrical current to separate the mol...
Gel Electrophoresis
Briefly summarize how gel electrophoresis is used to separate molecules - ✔️✔️when
the molecules are placed in the individual wells they are all at the same distance
because all the wells were created with the same comb. Gel electrophoresis is applying
an electrical current to separate the molecules. Once gel electrophoresis is ran, the
negative dyes will run towards the positive end of the gel and the positive dyes will run
towards the negative end of the gel. The heavier dyes will run a shorter distance
compared to the lighter dyes, this is how they are separated out.
What is agarose? - ✔️✔️it acts as a type of molecular strainer and prevents all the
molecules from moving too quickly. The electric current moves DNA molecules across
the agarose. The agarose gel is used to visualize the fragments. It can be used to
separate DNA molecules ranging from several hundred nucleotides in length to ober
10,000 nucleotides.
What are other techniques used to separate and identify molecules? - ✔️✔️distillation,
crystallization, liquid chromatography, gas chromatography, atomic absorption
Who invented gel electrophoresis? - ✔️✔️Oliver Smithies
Define the process of gel electrophoresis - ✔️✔️applying an electrical current to a
gelatin-like substance containing biological samples. When mixtures are placed within
the wells of the gel and an electrical current is applied , the molecules travel through the
gel and separate from one another according to each molecule's charge, size and
shape.
Define Gel Electrophoresis - ✔️✔️laboratory method used to separate mixtures of DNA
according to molecular size. Molecules are separated by being pushed through an
electrical field through a gel that contains small pores.
What is the agarose made from? - ✔️✔️an extract of red algae
What is the function of electrophoresis buffer? - ✔️✔️the buffer keeps the pH within a
narrow range. The charge is also transmitted via ions provided by the buffer.
What is the function of the wells in the gel? - ✔️✔️the sample being ran in gel
electrophoresis is loaded into the wells of the gel.
What is the function of the electric current? - ✔️✔️shocks it so that it will move towards
the positive and negative sides.
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